Efeito do extrato aquoso da bauhinia forficata link na prevenção do diabetes tipo 2 em modelo animal

• Ano de defesa: 2016
• Autor(a) principal: Gasparini, Patricia [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo
• Palavras-chave em Português: Decoction; C57BL/6J; STZ; Insulin signaling; Insulin-sensitive tissues; Decocção; Sinalização da insulina; Tecidos periféricos
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=4635833; https://repositorio.unifesp.br/handle/11600/46601

Diabetes mellitus (DM) is a chronic metabolic disease characterized by hyperglicemic and glucose intolerance, affecting about 415 million people in the world. The most common type of DM is the type 2 (T2DM), characterized by insulin resistance and/or relative insulin production deficiency. The adherence from society to phytotherapy has increased by many factors and they are being used in traditional medicine for treatment of several illness. The treatment for the DM can be performed through plant species in its decoction way, being Bauhinia forficata Link (Bf) one of the species utilized for this purpose. This plant is a potential source of natural antioxidants, such as the kaempferitrin, with hypoglycemic and antidiabetic activities. The isolated kaempferitrin was able to exhibit hypoglycemic effect on DM. However, the Bf studies could not elucidate its mechanism of action and only demonstrated its effect on DM treatment. This fact is staged on a global scenario of an increasing number of T2DM cases and high public health costs related to the disease. In this way, is necessary the development of studies which analyze the Bf preventive potential about TDM2 development, demonstrating the biological effects by its possible action mechanisms in insulin-sensitive tissues. In this sense, we aimed to evaluate the preventive effect of aqueous extract from Bf fresh leaves decoction in C57Bl/6J mice with streptozotocin (STZ)-high fat diet (HFD)-induced T2DM, analyzing pancreatic islets morphology and the signaling pathway of insulin in the liver, gastrocnemius skeletal muscle and retroperitoneal adipose tissue. The Bf extract used is a potential source of phenolic compounds and flavonoids and it was suggested the presence of kaempferitrin on it. In vivo, the Bf decoction was able to: 1) collaborate for the lower gain of body weight in the groups that used it, without altering the energy intake; 2) tend to improve glucose intolerance and partially prevent the development of insulin resistance; 3) reduce the enzyme pyruvic glutamic transaminase activity and the hepatic triglycerides, however, the same was not seen in the lipid parameters 4) and maintain the functional capacity of the pancreatic islets similar to the control, without altering its mass. The mechanism of action of Bf does not appear to occur in hepatic and adipose tissues, however it occurs in the muscle via pAKT/AKT. Therefore, the Bf decoction seems to partially prevent T2DM in the model used by pAKT/AKT in the gastrocnemius muscle.