Caracterização molecular de micobactérias ambientais e avaliação de degradação de hidrocarbonetos

• Ano de defesa: 2013
• Autor(a) principal: Uzam, Camilla de Paula Pereira [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo
• Palavras-chave em Português: Micobactérias não tuberculosas; Biorremediação; Hidrocarbonetos; Espectometria de massas; MALDI-TOF
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=89836; http://repositorio.unifesp.br/handle/11600/48713

The genus Mycobacterium has 165 species from which 157 belong to nontuberculous mycobacteria group (NTM). In spite of its medical interest, environmental mycobacteria started to gain attention due to their ability to degrade polycyclic aromatic hydrocarbons (PAHs). There is an urgent need for new techniques to rapidly identify mycobacteria isolated. This project approached two topics: the evaluation of mass spectrometry technique (MALDI-TOF) to rapid identify environmental mycobacteria and an examination of the potential of degradation of hydrocarbons by this group of microorganisms. We used a collection of mycobacteria reference strains and clinical isolates to generate the mass spectrometry sprectrum (MALDI-TOF) and we validated it against the Bruker Daltonics spectra database v3. One hundred twenty-five environmental isolates were identified using PRA-hsp65 and MALDI-TOF. The comparative analysis of results of PRA-hsp65 and mass spectrometry for identification of mycobacteria showed 100% agreement, but only eleven samples were identified at the level of species by the latter technique. The mass spectrometry method is promising but requires a representative database of spectra of reference samples, clinical and environmental, well characterized in order to provide reliability to the results obtained. Finally, it was possible to isolate, identify and characterize chemically one mycobacteria (MCVA038) which decomposes hydrocarbons of high molecular weight.