Prokineticina 2 recruta células da SVZ para o córtex lesionado em um modelo de traumatismo cranioencefálico em camundongos

• Ano de defesa: 2017
• Autor(a) principal: Mundim, Mayara Terra Villela Vieira [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Cranioencephalic injury; Prokineticina; Subventricular zone; Neural stem cells; Neurogenesis; Brain repair; Mice; Lesão cranioencefálica; Zona subventricular; Células tronco neurais; Neurogênese; Reparo cerebral; Traumatismos craniocerebrais; Camundongos
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5170395; http://repositorio.unifesp.br/handle/11600/50549

Traumatic brain injury (TBI) is an important cause of mortality and morbidity ali over the world. After the initial injury there is a cascade of cellular and molecular , events that ultimately lead to cell death. Therapies aim not only to counteract these mechanisms but also to replenish the lost cell population in order to achieve a better recovery. The adult mammal brain in not as plastic as the postnatal, but it has at least two neurogenic regions that maintains physiological functions in the brain; the subgranular zone of the dentate gyrus of the hippocampus, which produces neurons that integrate locally, and the subventricular zone (SVZ) of the lateral ventricles, that produces neuroblasts that migrate through the rostral migratory stream RMS) to the olfactory bulbs. Brain injuries, as well as neurodegenerative diseases, induce the SVZ to respond by increasing cell proliferation and migration to the injured areas. Here we report that SVZ cells migrate to the injured cortex after traumatic brain injury in mice, and that the physiological RMS migration is not impaired. We also show that Prokineticin 2 (PROK2, a chemokine important for the olfactory bulb neurogenesis by promoting the directional migration of neuroblasts, is induced in the injured cortex. Using PROK2 receptor antagonist and recombinant PROK2 we show that PROK2 can directionally attract SVZ cells in vitro and in vivo.