Efeitos da diacereína, do conjugado de glicosamina/condroitina e das células tronco derivadas de polpa dental na cartilagem articular do fêmur de ratos wistar com osteoartrite tese

• Ano de defesa: 2019
• Autor(a) principal: Maldonado, Diogo Correa [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Osteoarthritis; Articular Cartilage; Tooth Pulp Stem Cells; Glycosamine/Chondroitin; Diacerein; Wistar Rats; Osteoartrite; Cartilagem Articular; Células Tronco De Polpa De Dente; Glicosamina/Condroitina; Diacereína; Ratos Wistar
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=8755051; https://repositorio.unifesp.br/handle/11600/59779

The aim of the present study was to compare molecular and morphological effects of diacerein and glucosamine-chondroitin drug treatment and intra-articular injection of human deciduous dental pulp stem cells (hDPSC) therapy in rat femur with induced osteoarthritis (OA). Design: Thirty six male adult rats were randomly separated in six groups: Control Group (without induction of OA), Osteoarthritis Group 60 (induction of OA and euthanasia after 60 days), Osteoarthritis Group (induction of OA and euthanasia after 14 days), Diacerein Group, Glucosamine-Chondroitin Group, and Mesenchymal Stem Cells Group. The drug-treated groups were gavaged with 50 mg/kg of diacerein and 400/500 mg/kg of glucosamine-chondroitin for 60 days. The cell therapy treated group received an intra-articular single dose of 5x105 of human deciduous dental pulp mesenchymal stem cells. Lateral femoral condyles were collected and prepared for immunohistochemistry and light microscopy procedures. The morphological features (thickness and chondrocytes number) and immunoexpression of SOX-5, IHH, MMP-8, MMP-13, and Collagen 2 were statistically analyzed. Results: Our data suggest that hDPSC therapy contribute more actively and effectively in lateral femoral condyles structure reorganization (collagen 2, thickness, number of chondrocytes and cell hypertrophy - IHH). In contrast, the glucosamine-chondroitin sulfate was more effective in inflammatory control (MMP-8 and MMP-13), while diacerein showed better results associated with the maintenance of the primordial cartilage (SOX-5). Conclusion: We may confirm positive therapeutic effect of daily-administered conventional drugs in rat model of OA. However, one single dose of locally administrated hDPSC provides significant improvement in tissue regeneration in OA.