Ação dos esteroides ovarianos sobre a quantidade dos glicosaminoglicanos sulfatados e do ácido hialurônico nas mamas de ratas ovariectomizadas

• Ano de defesa: 2016
• Autor(a) principal: Torres, Sueli Maria Preda dos Santos [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Breast; Oestradiol; 17-acetate of medroxyprogesterone; Glycosaminoglycans; Rats; Glicosaminoglicanas; Mama; Estrogênios; Progesterona; Ratas
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=4016967; http://repositorio.unifesp.br/handle/11600/46349

The extracellular matrix (ECM) act an essential role in the function of mammary epithelial cells and glycosaminoglycans have long been attracting clinical interest in all areas of medicine for their important role in cell recognition, migration, cell proliferation and differentiation. OBJECTIVE: Evaluate the morphology and the concentration of glycosaminoglycans in the breast of female rats treated with estrogen and/or progesterone. MATERIAL AND METHODS: Sixty female rats were divided into six groups with 10 animals each: G1 (5 months) and GII (18 months) in the proestrus phase were treated with vehicle; the other animals were ovariectomized, and immediately after were treated with vehicle (GC) or with hormones ? (GE) treated with oestradiol benzoate (37.6 mg/animal); (GP) ? treated with medroxyprogesterone acetate (11.28 mg/animal) and (GEP) ? treated with oestradiol benzoate (37.6 mg/animal), more medroxyprogesterone acetate (11.28 mg/animal). In the GE group, the oestradiol was administered subcutaneously for seven days; in the GP group, the progestogen was administered subcutaneously for 23 dats; in the GEP group, the oestradiol was administered once a day in the first seven days and the progestogen throughout the next 23 days, subcutaneously. Twenty four hours after the last administration of hormones, the animals were euthanized, and the first pairs of inguinal breasts were removed, part of them being fixated in formaldehyde at 10% (morphological evaluation) and the other part submerged in acetone (evaluation of the glycosaminoglycans). The material destined to the morphology was included in paraffin, and the cuts were stained by hematoxylin-eosin. The material destined to the biochemistry was processed to the electrophoresis in agarose gel to sulfated GAGs analysis, and the hyaluronic acid was analyzed with a Elisa-Like fluorimetric method. The statistical analysis was made by the analysis of variance (ANOVA) followed by the Bonferroni test (p<0.05). RESULTS: The breasts of the groups with ovaries (GI and GII) as well as the ovariectomized control group (GC) have shown to be atrophic, wherein we observed typical alveoli with secretion in the lumen in the groups (GE) and (GEP); yet in the animals treated with progestogen (GP), we noticed alveoli formed basically by cells that occupied almost all the alveolar light. In relation to the data of the sulfated glycosaminoglycans we noticed higher concentration in the oophorectomized group (GC) due to high concentration of dermatan sulfate (GC = GE > GP = GEP > GII = GI; p<0.05). And in relation to the hyaluronic acid we found higher concentration in the animals of five months (GI > GII, GC, GE, GEP > GP; p<0.005).CONCLUSION: Oestradiol and progesterone had a trophic effect in the breast parenchyma. However, the administration of oestradiol beforehand changes the action of progesterone in the rat?s mammary tissue.