Videomonitoração de longa duração (1 ano) de fêmeas que não desenvolveram status epilepticus pós pilocarpina e a possível ocorrência de crises espontâneas tardias

• Ano de defesa: 2017
• Autor(a) principal: Dal Pai, Janise [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Epilepsy; Female rats; Status epilepticus; Videomonitoring; Ovariectomy; Epilepsia; Fêmeas; Videomonitoração; Ovariectomia
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5454213; http://repositorio.unifesp.br/handle/11600/50033

Introduction: Epilepsy is one of the main neurologic diseases and Temporal Lobe Epilepsy (TLE) is the most frequent form. TLE affects the minimum of 20% of all patient with epilepsy and equally around 50 million of man and woman in the whole world. The use of the experimental model of epilepsy by administering the cholinergic agonist Pilocarpine (Pilo) mimics most of the behavioral and electrographic alterations found in TLE. Female rats submitted to this model allow the study of the hypothalamic-pituitary-gonadal axis alterations due to epileptic seizures. Studies in our laboratory has shown that 47% of the whole female rats who has received Pilo did not develop status epilepticus (SE). Navarro et al. (2009) found that male rats that did not develop SE (NSE) after receiving Pilo showed up spontaneous and recurrent seizures (SRS) starting 6-8 months after treatment with the drug. The study of TLE in ovariectomized (OVX) and non ovariectomized female rats (NOVX) induced to Pilo model that did not develop SE may show up important informations about the mechanisms involved in the generation and in the absence of SE in female rats, also the physiopathology of Pilo experimental model and the hormonal neuroprotection in the physiopathology of epilepsy. Aims: To investigate whether NOVX and OVX female rats, before or after receiving Pilo, and did not have SE, will develop SRS in the following one-year videomonitoring period. Methods: OVX and NOVX female rats (with regular cycle) received 360 mg/kg of Pilo ip. and were distributed in 6 experimental groups NSE, NSEOVX, OVXNSE, SE, SEOVX and OVXSE. Female rats were videomonitored for 12 months for the observation of SRS. The video acquired along this period were analyzed in fast speed and after that in real speed (speed in which the movements occur) in search of motor alterations. Results: Female rats from SE, SEOVX and OVXSE groups had their recordings watched in the fast speed, which allowed the observation that all of them became epileptic. Some females have even showed seizure upper to stage 5 from Racine (1972) and were classified as class motor seizures by Pinel e Rovner (1978a e 1978b). No motor alterations could be verified in the NSE, NSEOVX and OVXNSE females when recordings were analyzed in the fast speed. However, when the real speed was used, it was possible to identify very similar behaviors described as stages 1-3 from Racine (1972) and were classified as suggested seizures. Besides these, other behaviors not yet described in the literature for animals injected with Pilo were observed, however some of them are very similar to motor alterations seen in epilepsy patients. These behaviors were also identified in SE, SEOVX and OVXSE female groups. Considering the findings of this thesis, it is suggested a complement to be added to the classifications already known for seizures in rats named FeBAP Classification, which may be used in female rats that developed or not SE after being induced to Pilo model. Conclusion: Watching the videomonitoring in the real speed was essential for identifying motor alterations in NSE, NSEOVX and OVXNSE female groups. The similarity among observed manifestations, those described as stages 1-3 and behaviors found in SE, SEOVX and OVXSE female groups suggest that those are seizures with origin in a possible epileptogenic focus. Videomonitoring was fundamental for identifying motor alterations however, there is a need of an electroencephalographic study to confirm the findings from this work.