Hidroxiapatita nano e microparticulada: interação óssea

• Ano de defesa: 2019
• Autor(a) principal: Segala, Renato Dalcin [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Hydroxyapatite; Tissue Engineering; Biomaterials; Hidroxiapatita; Engenharia Tecidual; Biomateriais
• Link de acesso: https://repositorio.unifesp.br/handle/11600/58420

Introduction: Tissue Engineering (ET) is the science that associates biological knowledge with Engineering fundamentals. ET is based on the use of cell-seeded biocompatible materials (scaffolds) that have the ability to differentiate into tissues. Among these biomaterial, hydroxyapatite (HA) is the most studied for ET of mineralized tissues. The evaluation of the biomaterial of HA is fundamental to collaborate with the bone regeneration. Aim: To evaluate the interaction of hydroxyapatite implants nano and microparticulate in rabbit tíbia. Methods: Fifty-six hydroxyapatite implants was implanted on twenty- eigth rabbit tíbia. 28 implants was nano particulate hydroxyapatite and 28 micro particulate hydroxyapatite, 14 nano and micro particulate implants was sintered by ultrasound (US) and 14 sintered by magnetic stirring (MS). Three holes were made in the tibia of the rabbits, in the proximal holes were implanted the nano particulate biomaterials (positive control), in the central holes were implanted the micro particulate (experimental sample) and, the distal orifice was empty (negative control). Three radiographic controls were performed with a 14-day interval. After 28 days the painless induced death was performed and the specimens were harvested and submitted to histological analysis and MicroCT. Results: The positive controls MS and US presented lower bone growth and greater porosity in relation to the experimental samples, whereas the negative controls had higher bone growth and lower porosity. Conclusions: The use of a nano particulate hydroxyapatite biomaterial promotes greater bone growth with lower porosity, collaborating with ET of mineralized tissues.