Alterações promovidas pela caquexia associada ao câncer sobre a inervação do tecido adiposo de pacientes caquéticos

• Ano de defesa: 2016
• Autor(a) principal: Cavalaro, Diego Alexandre [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Cachexia; Cancer; Mesenteric adipose tissue; Cytokines and sympathetic nervous system; Caquexia; Câncer; Tecido adiposo mesentérico; Citocinas; Sistema nervoso simpático
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=4628174; https://repositorio.unifesp.br/handle/11600/46378

Introduction: Cachexia is a multifactorial syndrome, characterized by loss of skeletal muscle tissue and / or adipose tissue, which cannot be completely reversed even with nutritional support, and is often associated with advanced stages of cancer. The sympathetic nervous system (SNS) may have a role in the development of this syndrome by controlling lipid metabolism through catecholamines. Objective: To analyze possible differences in sympathetic innervation, adrenergic receptors in the inflammatory process in the mesenteric adipose tissue of patients with cancer cachexia , non-cachectic and controls. Methods: The study involved 87 patients, recruited from the University Hospital of the University of São Paulo, classified into 3 groups: Control, cancer without cachexia (WSC) and cancer cachexia (CC). Blood samples were collected for biochemical measurements. Mesenteric adipose tissue samples employed used for determination of cytokines, gene expression, protein concentration, expression of adrenergic receptors and to analyze the frequency of adrenergic fiber immunoreactivity for anti-tyrosine hydroxylase. Results: CC showed greater weight loss, anemia, hypoalbuminemia, higher concentrations of C-reactive protein (CRP) and lower score ( p<0.0001 ) for quality of life. CC also showed higher plasma concentrations of interleukin-6 (IL-6) and tumor necrosis factor-? (TNF-?). Significant differences in gene expression and protein quantification of adrenergic receptors in mesenteric adipose tissue were not observed. The qualitative analysis of the density of adrenergic fiber in the mesenteric adipose tissue was not different between groups. Conclusions: CC patients showed a inflammatory profile presenting higher plasma concentrations of pro inflammatory cytokines and PCR. No changes were found for gene expression and protein quantification of adrenergic receptors in mesenteric adipose tissue of patients with cancer cachexia, nor weight- stable counterparts. Conclusion: The results suggest that the development of cachexia is independent of changes in the gene expression and protein quantification of alpha and beta adrenergic receptors, as well as changes in the presence of adrenergic fibers, in the mesenteric adipose tissue.