Avaliação dos efeitos da privação alimentar sobre o condicionamento ambiental induzido por diferentes drogas de abuso

• Ano de defesa: 2017
• Autor(a) principal: Hollais, Andre Willian [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Food deprivation; Conditioned place preference; Ethanol; Nicotine; Morphine; Femproporex; C-Fos; Orexin; Saúde mental; Reforma psiquiátrica; Análise institucional; Oferta e demanda; Analisadores
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5557652; http://repositorio.unifesp.br/handle/11600/50076

Cumulative experimental evidence points to the existence of positive correlation between the exposure to different stressors and increased vulnerability to addiction. There is a need to search for stress markers which more reliably reflect the human condition, in laboratory animal models. Among them food deprivation has been considered. Indeed, it has been demonstrated that food deprivation, followed by exposure to different drugs of abuse is able to enhance and/or facilitate drug dependence in animal models, including the conditioning place preference (CPP) paradigm.In this scenario, the aim of the present study was to investigate the tardive effects of diurnal food deprivation on CPP induced by different drugs with potential for abuse, the front mechanistic approaches, pharmacological and immunohistochemistry. For Swiss mice adult males were deprived of daytime power for 4 days. After 21 days of the end of food deprivation, the animals were conditioned to ethanol, morphine, nicotine or femproporex and submitted to the postconditioning test to evaluate the CPP. Our results indicate that daytime food deprivation was able to induce a greater resistance to extinction CPP ethanol, and potentiate the expression of nicotine-induced CPP and fenproporex. However, seems not to have altered sensitivity to morphine conditioning. The immunohistochemical point of view, the daytime food deprivation protocol was able to induce greater immunoreactivity to c-fos protein in the nucleus accumbens, basolateral amygdala, lateral hypothalamus and ventral tegmental area, immediately after the food deprivation in However, this increased expression was not observed 7, 14 and 21 days after the end of food deprivation. However, the pharmacologically treatment with SB-334867, applied simultaneously to food deprivation regimen was able to prevent the facilitating effects of starvation on the conditioned place preference induced fenproporex. Taken together, our results suggest that daytime food deprivation was able to increase vulnerability to rewarding effects of nicotine and fenproporex, or increase resistance to ethanol extinction of conditioned behavior. However, it seems to have influenced the conditioning to morphine. At the same time, by immunoreactivity to the protein c-fos was evidenced the effective participation of the nucleus accumbens, basolateral amygdala, lateral hypothalamus and ventral tegmental area in the effects of starvation on the place conditioning for different drugs with abuse potential. Furthermore, pharmacological and mechanistic point of view, the administration of the antagonist OX1R receiver, simultaneously to the protocol food deprivation was able to prevent the facilitatory effect of starvation on conditioning a place induced fenproporex, which suggests not only effective participation orexinérgico system in the genesis of such effects, but also seems to reveal the potential therapeutic benefit of SB-334867 in the prevention of the effects of food deprivation on the disorder related to substance use. .