Estudo do efeito da manipulação da rede de citocinas sobre o padrão de sono e desempenho de camundongos na tarefa de esquiva inibitória de múltiplas tentativas

• Ano de defesa: 2016
• Autor(a) principal: Esumi, Lia Assae [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: IL-12; IL10; IL-10; Interleucina; Memória; Eletrocorticograma
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3629049; http://repositorio.unifesp.br/handle/11600/47035

Among the many functions already related to sleep, the relationship of this phenomenon with the memory processing is probably one of the most controversial issues in neuroscience. In attempt to obtain further clarification on this issue, this study aimed to modulate the sleep pattern of mice with the objective of analyze possible changes in memory. To modulate the sleep pattern we used a class of molecules that endogenously participates in sleep regulation ? the cytokines. The anti-inflammatory cytokine interleukin-10 (IL-10) and the pro-inflammatory cytokine IL-12 were administered intraperitoneally into Swiss mice in attempt to modulate the inflammatory profile. There were three purposes: (1) verify the effect of IL-10 and IL-12 treatments in the sleep pattern, (2) verify the effect of IL-10 and IL-12 treatments in the sleep pattern along with the performance in a memory task, and (3) identify possible correlations between sleep pattern and performance on the memory task. To evaluate the sleep pattern, the animals underwent a surgery to implant electrodes for eletrocorticogram and electromyogram recordings. Memory was evaluated through the multiple-trial inhibitory avoidance (MTIA) task. The MTIA task training was performed immediately after treatments, and animals were tested 24h later. Alone, IL-10 and IL-12 treatments did not induce changes in sleep pattern. However, when treatments were conducted immediately before the MTIA task training, the IL-12 group showed decrease in the number of wake, slow-wave sleep (SWS), and paradoxical sleep (PS) episodes; increase in the mean duration of wake episodes; and decrease in the mean duration of PS episodes. Such sleep/wake changes were accompanied by performance improvement of IL-12 group in the EIMT task test. The correlations between the performance on MTIA task and the sleep/wake parameters in the post-training period indicated the contribution of SWS in the first three hours after training, followed by the greater contribuition of the complete cycle, wake? SWS? PS, for the best performance in the EIMT task. We suggest that the superior performance of the IL-12 group is the result of optimization of the memory consolidation process through changes in sleep patterns induced by IL-12 treatment.