Análise de marcadores imuno-histoquímicos de identificação dos tumores de células germinativas do testículo e sua interferência na espermatogênese

• Ano de defesa: 2021
• Autor(a) principal: Xavier, Rosana [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo
• Palavras-chave em Português: Neoplasias testiculares; Seminoma; Não-seminoma; Espermatogênese; Marcadores tumorais
• Link de acesso: https://hdl.handle.net/11600/63568

Objective: To analyze whether overexpressed proteins in seminal plasma of men af-fected with testicular germ cell tumor (TGCT) can also predict altered levels in testicu-lar tissue in areas affected by TGCT and non tumoral, and if there is a difference in this expression between seminomatous and non-seminomatous tumor subtypes. Methods: We compared the intensity of different markers (GP2, GSH2, CD14, APOA1 and B4GALT) quantified by immunohistochemistry, and their relationship with different tissues (seminoma, non-seminoma and non tumoral/control) through multinomial and simple logistic regression. Results: The B4GALT1 marker showed a significant difference between non-tumor and non-seminoma tissues (p < 0.01); the GSH2 marker, among the different proteins analyzed, was the only one that was sta-tistically significant to predict the tissue type among the three comparisons, manag-ing to differentiate seminoma from non-seminoma (p < 0.01) and non-tumor tissue from seminoma (p < 0.01); the GP2 marker was statistically significant between non-tumor tissues and both tumors, seminoma and non-seminoma (p < 0.001); the APOA1 marker showed difference only between non-tumor tissue and seminoma (p < 0.001); the CD14 marker did not obtain a significant result, as well as the analysis of the markers considering the variables analyzed by HE. Conclusion: Although the analyzed markers APOA1, B4GALT1, CD14, GP2 and GSH2 were not able to predict fertile potential they were considered possible biomarker proteins, with specificity in differentiating peri-tumoral tissue, seminoma and non-seminoma.