Avaliação da cartilagem articular do joelho de ratos com artrite induzida e tratados com curcumina

Detalhes bibliográficos
Ano de defesa: 2019
Autor(a) principal: Maria, Tiago Nicoliche [UNIFESP]
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=7584110
https://repositorio.unifesp.br/handle/11600/59759
Resumo: This study was designed to evaluate the anti-inflammatory effects of curcumin in the induced osteoarthritis treatment in rats’ knee. Design: Fifteen adult rats were used and sectioned in three groups: Osteoarthritis Group (OAG), Control Group (CG – without induction of osteoarthritis), and the Curcumin Osteoarthritis Group (COAG). Osteoarthritis was induced in the right knee of OAG and COAG by an intra-articular injection of 1 mg Zymosan. Fourteen days after induction, 50 mg/kg of Curcumin was daily administered for 60 days by gavage to the COAG. After 8 weeks, rats from all groups were euthanized. Medial femoral condyles were collected for fixation, decalcification, and immunohistochemical and light microscopy procedures. The expression of the antibodies SOX-5, IHH, MMP-8, MMP-13, and Collagen 2 (Col2) were analyzed. Results: COAG demonstrated an increase in the number of chondrocytes in the surface and middle layers when compared with OAG and CG, respectively. COAG also showed a decrease in the thickness of the middle and deep layers when compared with OAG and an increase in the Col2 expression was found in all the articular layers (surface, middle, and deep) in the COAG when compared with OAG. There was an increase in the SOX-5 expression in the surface and deep layers of the COAG when compared with OAG and CG, respectively. Conclusion: The results indicated that the treatment with curcumin demonstrated neither an improvement in cartilage thickness nor in MMP-8 and MMP-13 expressions, but it showed improvement in IHH, Col2, SOX-5 expressions and in the number of chondrocytes.