Alterações histopatológicas e imuno-histoquímicas na poiquilodermia de Civatte após tratamento com luz intensa pulsada

• Ano de defesa: 2009
• Autor(a) principal: Scattone, Luciane [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Colágeno; Imuno-histoquímica; Melaninas; Tecido elástico; Fototerapia
• Link de acesso: http://repositorio.unifesp.br/handle/11600/8883

Objective: To assess through histological and immunohistochemical analyses the cell changes after Intense Pulsed Light therapy in the cervical region of patients with poikiloderma of Civatte. Methods: Fourteen patients with poikiloderma of Civatte in the cervical region were studied. They were submitted to three monthly sessions of Intense Pulsed Light. Biopsies were performed before and after treatment. Five slides were prepared for each patient summing up 70 slides for histological and immunohistochemical analyses. The slides were stained with hematoxilyn-eosin (HE), Masson trichrome (for colagen), Verhoeffvan- Gieson (for elastic fibers), Fontana-Masson (for melanin) and CD 34 immunohistochemistry. Results: Improvement in both skin pigmentation and texture was observed in all patients in Masson Trichrome staining, showed evident proliferation and compaction (neocollagenesis) of collagen fibers in the papillary dermis, which was confirmed by digital imaging analysis. By Fontana- Masson staining even distribution of melanin pigment was found; the mottled pattern improved. In slides stained by Verhoeff-van-Gieson, the elastic fibers increase in number, mainly in the cases with higher collagen density. In Immunohistochemistry, there were no significant alterations in vessels after Intense Pulsed Light therapy. Conclusions: A significant increase in density and intensity of collagen fibers, increase in number of elastic was observed in papillary dermis and redistribution of melanin when comparing the groups before and after treatment with Intense Pulsed Light. The vessel diameter was smaller in five patients (35,7%).