Secretoma e interactoma comparativo de Trypanosoma cruzi e Trypanosoma rangeli

• Ano de defesa: 2017
• Autor(a) principal: Costa, Renata Watanabe [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Chagas disease; Secreted proteins; Cerâmicas eletrônicas; Ccto dopado com Mg; Propriedades dielétrico; Doença de Chagas
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5098372; http://repositorio.unifesp.br/handle/11600/50658

Chagas disease, a zoonosis caused by the flagellate protozoan Trypanosoma cruzi, is a chronic and systemic parasitic infection that affects approximately 8 million patients in Latin America, being an emerging public health problem due to the lack of immunization and effective chemotherapy. According to recent studies, there are several T. cruzi secreted proteins that interact with the human host during cell invasion. Moreover, several comparative studies with T. rangeli, non-pathogenic for humans, have been performed in order to identify proteins directly involved in the pathogenesis of the disease. Regarding identification and large-scale protein characterization, bioinformatics has grown significantly with the evolution of specific computational softwares for proteins prediction. In this study, we present an integrated computational network in order to analyze secreted proteins of both species. Additionally, we propose an interactome and phylogenetic trees of some selected proteins. In T. cruzi, we identified 463 exclusively secreted proteins; compared to 202 in T. rangeli. Among the secreted proteins identified in T. cruzi, we found several trans-sialidases, mucins, MASPs, proteins with phospholipase 2 domain (PLA2-like) and Hsp70 domain (Hsp70-like) which were previously characterized by other studies and were demonstrated to be related to T. cruzi virulence. Proteins found in T. rangeli were related to protozoan metabolism as carboxylases and phosphatases. Furthermore, there were also proteins that may interact with the human's immune system such as heat shock and MASP proteins, but in a smaller number compared to T. cruzi. Finally, another selected protein, HYOU1, an orthologous human protein to the identified Hsp70-like protein, present in both species, showed interaction with other proteins of the immune system. These results will pave the way for a better understanding of the pathophysiology of Chagas disease and ultimately leading to the identification of molecular targets for drug development.