Expressão gênica de marcadores da imunidade em queratinócitos cultivados de pacientes com grande queimadura

• Ano de defesa: 2016
• Autor(a) principal: Corrêa, Silvana Aparecida Alves [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Expressão gênica; Imunidade; Queratinócitos; Queimadura vias de sinalização
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=4143877; http://repositorio.unifesp.br/handle/11600/48489

INTRODUCTION: Burns affect about one percent of the world population. More than one million burns occur in the United States each year and about 5,000 are fatal, causing the burn is the fourth leading cause of death from unintentional injuries in this country. Victims of burns have high susceptibility to infections, being directly related to morbidity and mortality rates. PURPOSE: To evaluate genic expression of immunity markers in cultivated keratinocytes of patients with large burn. METHODS: After obtaining viable fragments of skin with and without burning, culture of keratinocytes was initiated by the enzymatic method using Dispase. These cells were treated with Trizol® for extraction of total RNA. This was quantified and analyzed for purity for obtaining cDNA for the analysis of gene expression using PCR Arrays plates for immunity. RESULTS: After the analysis of gene expression we found, in keratinocytes culture, seven (07) differentially expressed genes (8%) that presented 100% hipo-expressed. And, in skin fragments, 63% genes were differentially expressed, of which 77% were repressed and 23% were hyper-regulated. CONCLUSION: In the acute phase in large burn were observed HLA-E, IL1R1, and IL-6 been priority genes in the keratinocyte culture; and in the skin fragments the priority genes were IL-8, IL-6, TNF- alfa, HLA-E, LYZ, and CCR6.