Ação de um Peptídeo Derivado do Lopap em Cultura de Fibroblastos e em Modelo Experimental de Lesão Cutânea em Ratos

• Ano de defesa: 2009
• Autor(a) principal: Wlian, Luana [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Collagen; Glycosaminoglycans; Metalloproteinases; Tissue repair; Lonomia obliqua; Colágeno; Glicosaminoglicanos; Metaloproteinases; Reparação tecidual
• Link de acesso: http://repositorio.unifesp.br/handle/11600/8863

The process of tissue repair is a complex of reactions between cells and biochemical mediators that are triggered from an injury. This study evaluated the effect of peptide 4 derived from the amino acid sequence of Lopap (Lonomia obliqua prothrombin activator protease) in culture of fibroblasts to evaluate the expression of molecules of extracellular matrix and the membrane receptors of interleukin-8 and in experimental model of skin lesion in rats to examine the effect of the molecule in the process of tissue repair. We used 40 male Wistar rats with 6 to 8 weeks, randomized to 5 experimental groups. After anesthesia, the rats were submitted to surgery, where eight circular full-thickness of skin from 6 mm of diameter were surrounded in the back and treated with saline or with peptide 4. After different periods (days 3, 7, 14 and 21 days after wounding) were carried out tests to evaluate the process of tissue repair between the groups. We conclude that peptide 4 is able to stimulate fibroblasts in culture and promote the acceleration of the process of tissue repair in vivo model with greater deposition of collagen, glycosaminoglycans and better tissue reorganization.