Histologia, função coclear e genotoxicidade em cobaias tratadas com cisplatina

• Ano de defesa: 2011
• Autor(a) principal: Franceschi, Cacineli Marion de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Santa Maria; BR; Fonoaudiologia; UFSM; Programa de Pós-Graduação em Distúrbios da Comunicação Humana
• Palavras-chave em Português: Ototoxicidade; Genotoxicidade; Cisplatina; Cobaias; Histologia; Cóclea; Ototoxicity; Genotoxicity; Cisplatin; Guinea pig; Histology; Cochlea; CNPQ::CIENCIAS DA SAUDE::FONOAUDIOLOGIA
• Link de acesso: http://repositorio.ufsm.br/handle/1/6490

The present work aims to investigate the cisplatin influence on the cochlea and the deoxiribonucleic acid (DNA) of guinea pigs. An experimental study was executed with 12 guinea pigs (Cavia porcellus). The inclusion criterion for guinea pigs in the sample was the presence of Preyer's reflex (contraction of the pinna when facing sound stimulus) and distortion product otoacoustic emissions (DPOAEs). Guinea pigs were divided into two groups: Control Group (CG) - composed by six guinea pigs, to which it was administrated physiological solution of sodium chloride 0.9% during six consecutive days; Study Group (SG) - composed by six guinea pigs to which it was administrated cisplatin in six consecutive doses of 3mg/kg/day intraperitoneally. Twenty-four hours after the last cisplatin injection, guinea pigs were sacrificed, the blood sample was collected to perform the Comet Essay, and the cochleas were removed to histological analysis. When comparing the SG guinea pigs before and after the cisplatin administration, it was verified a statistically significant reduction of the amplitude of DPOAEs, mainly in the frequencies of 1000Hz to 3998Hz. After the cisplatin administration, it was certified that the amplitude of the DPOAEs frequencies of 2830Hz and 5657Hz from the SG guinea pigs suffered a statistically significant reduction compared to the CG guinea pigs. After the Cisplatin administration there were not detected any identifiable DNA changes in the Comet essay, the histological analysis showed alterations in the organ of Corti and in the spiral ganglion. Cisplatin causes alterations in the function and cochlear morphology, however, any damage to the DNA was detected.