Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum)

• Ano de defesa: 2023
• Autor(a) principal: Santos, Izaura Ceolin dos
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Santa Maria; Brasil; Ciência e Tecnologia dos Alimentos; UFSM; Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos; Centro de Ciências Rurais
• Palavras-chave em Português: Colorimetria; Imagens digitais; Determinação de eugenol; Óleo essencial de cravo-da-índia; Colorimetry; Digital images; Eugenol determination; Clove essential oil; CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
• Link de acesso: http://repositorio.ufsm.br/handle/1/29097

In this study, innovative and fast alternatives for determining the major constituents of clove essential oil were developed with digital images. The experiments were initially carried out using thin-layer chromatography (TLC) and, later, by colorimetry using a smartphone. The types of eluents, lighting, software, development methods, detection, and the influence of volume on the samples were previously optimized for TLC. A portable system was designed to acquire images based on the use of a smartphone, with subsequent quantification tests being made using the Photometrix app and other free software such as Image J and qTLC. The results were inaccurate due to the lack of reproducibility in analyses. Thus, colorimetry associated with the use of digital images in a smartphone was used for the determination of eugenol in clove oil. The Photometrix app was used to evaluate the colorimetric reaction between the eugenol and the Folin-Ciocalteu reagent. The results were in agreement with those obtained through traditional methodologies, such as gas chromatography and spectrophotometry, ranging from 97% to 101% for the five samples tested. The developed method allowed the analysis of several samples in a short period of time. Portability and ease of operation are also noteworthy, making it useful in the field and in places with restricted access.