Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum)
Ano de defesa: | 2023 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Brasil Ciência e Tecnologia dos Alimentos UFSM Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos Centro de Ciências Rurais |
Programa de Pós-Graduação: |
Não Informado pela instituição
|
Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/29097 |
Resumo: | In this study, innovative and fast alternatives for determining the major constituents of clove essential oil were developed with digital images. The experiments were initially carried out using thin-layer chromatography (TLC) and, later, by colorimetry using a smartphone. The types of eluents, lighting, software, development methods, detection, and the influence of volume on the samples were previously optimized for TLC. A portable system was designed to acquire images based on the use of a smartphone, with subsequent quantification tests being made using the Photometrix app and other free software such as Image J and qTLC. The results were inaccurate due to the lack of reproducibility in analyses. Thus, colorimetry associated with the use of digital images in a smartphone was used for the determination of eugenol in clove oil. The Photometrix app was used to evaluate the colorimetric reaction between the eugenol and the Folin-Ciocalteu reagent. The results were in agreement with those obtained through traditional methodologies, such as gas chromatography and spectrophotometry, ranging from 97% to 101% for the five samples tested. The developed method allowed the analysis of several samples in a short period of time. Portability and ease of operation are also noteworthy, making it useful in the field and in places with restricted access. |