Avaliação da atividade biológica in vitro da amitriptilina

• Ano de defesa: 2019
• Autor(a) principal: Machado, Catrine de Souza
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Santa Maria; Brasil; Farmacologia; UFSM; Programa de Pós-Graduação em Ciências Farmacêuticas; Centro de Ciências da Saúde
• Palavras-chave em Português: Amitriptilina; Compostos antibacterianos; Reposicionamento de drogas; Clivagem do DNA; Amitriptyline; Antibacterial compounds; Repositioning drugs; DNA cleavage; CNPQ::CIENCIAS DA SAUDE::FARMACIA
• Link de acesso: http://repositorio.ufsm.br/handle/1/20377

Theemergenceofinfectionscaused bymulti-drug resistantbacteriaworldwideand thesubstantialincreasein cancerhasled tothesearchforinnovativetherapies.Inthisway,theuseofresearcheswithdrugsalreadyinclinicalusethatcould providemoreeffectivetreatmentalternativesto overcomethesecurrentchallenges,dueto thelimited numberofavailabletherapeuticagents,characterizing therepositioning orredirectionofdrugs,wasused.Amitriptylineisatricyclicantidepressantnotwidelyused intheclinicforthispurposebecauseofitsmarked sideeffectsinthedoseat whichithasto beused.However,atalowerdose,itsuseiscurrentlyinthetreatmentofpain,suchasprophylacticmigraineand cancerpain.However,studieshavedemonstrated theiractivityagainstvarioustypesofbacteriaand fungi.Duetoitsdiversified biologicalactivitycoupled withtherelativelylow priceand theisolation ofmultiresistantbacteria(MDR)mainlyatthehospitallevel,wewereencouraged to conductthisresearch.Oftendrugsthat arecapableofcleaving DNAdouble-stranded haveboth antibacterialand anti-cancereffects.Thisknowledgeand theexistenceofonlyhypothesesin theclarificationofthemechanismofactionofthisdrugistheobjectiveofthisstudyinwhich weintend to evaluatetheinvitrobiologicalactivityofthedrugamitriptylineagainstAmerican Standard CultureCollection (ATCC)referencestandard strainsand clinicalisolatesMDR,bydetermining theminimuminhibitoryconcentration(MIC),minimumbacterialconcentration (MBC),tolerancelevel,fractionalinhibitoryconcentrationindex(FICI),associated ornot with antibioticsused inclinicalpracticeand cleavageabilityofthepUC18 doublestranded plasmidDNA.