Desenvolvimento de modelo experimental de mielorradiculopatia esquistossomótica

• Ano de defesa: 2014
• Autor(a) principal: Carvalho, Tiago Pinheiro Vaz de
• Orientador(a): Araújo, Karina Conceição Gomes Machado de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Biologia Parasitária
• País: BR
• Palavras-chave em Português: Neuroesquistossomose; Schistosoma mansoni; Esquistossomose; Medula espinhal; Hiperalgesia
• Palavras-chave em Inglês: Neuroschistosomiasis; Schistosoma mansoni; Animal; Hyperalgesia
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA
• Link de acesso: https://ri.ufs.br/handle/riufs/3242

Schistosomalmyeloradiculopathy (SMR) is a severe formofpresentationof schistosomiasis, in whichthe spinal cord isaffectedby species ofSchistosoma. Thisstudy aimed todevelopan animal modelof SRMcaused byS.mansoni, and characterize the sensory and motorchanges causedby the depositionofeggsof S. mansoniin the spinal cordand correlate thesensory aspects, engines and histologicalanimalsinfunction of time.Heldmechanicalsensitivitytests, behavioral and subsequenteuthanasiain two series(infected and control)animalsdividedinto six groups offivemale Wistar ratseachin5, 10, 20 and 30dayspost-infection. The experimental groupswere anaesthetizedwith halothane andinjected witha suspension ofS. mansonieggs in thesubarachnoid space.Control animalsunderwent the sameprocedure, but with phosphate buffersolution administration(PBS). The spinal cordwas removed,subjected tohistologicalanalysis ofT10and L5. It was observedreduction in surfacemechanical sensitivity, thermal sensitivityand muscle strengthcaused by theeggsof S. mansoniin the spinal cord; Changesobservedin nervous tissueof 25% of the animals following infectionof S. mansoni. The modelproved to besuitable forevaluation offunctional changesin ratsand may beuseful forpathogenesisstudiesofMRE.