Estabelecimento de modelo murino para neurotuberculose experimental

• Ano de defesa: 2015
• Autor(a) principal: Dalan, Camilla de Aguiar
• Orientador(a): Lucca Junior, Waldecy de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Biologia Parasitária
• País: Brasil
• Palavras-chave em Português: Neurotuberculose; Modelo experimental; Desenvolvimento
• Palavras-chave em Inglês: Neurotuberculosis; Experimental model; Development
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::PARASITOLOGIA
• Link de acesso: https://ri.ufs.br/handle/riufs/3250

The occurrence of tuberculosis (TB) reaches much of the world's population and the prevalence is accompanied by the World Health Organization (WHO) since 1990. The same organization publishes the overall situation of the disease, as well as the situation of countries where the disease is not under control. Moreover, in recent years the number of people infected with Mycobacterium tuberculosis grows for many reasons, especially those derived from social inequality, AIDS, migration and the emergence of resistant bacilli to drugs. Moreover, the infection in the central nervous system (CNS) the bacillus is one of the most devastating clinical manifestations of the disease. This is called extrapulmonary neurotuberculosis (Ntb) and stands out with a high rate of mortality and morbidity among the extrapulmonary manifestations. The risk factors include age Ntb, coinfection with HIV, malnutrition, children with recent measles, alcoholism, malignant diseases, the use of immunosuppressant‟s in adults and in community prevalence of the disease. However, pathophysiological mechanisms of the disease need to be better understood. In this respect, more research is urgently needed to better understand the disease and to study new vaccines against the disease. Methods: On day 0 C57BL / 6 mice were challenged in the CNS of the lateral ventricle with micro stereotactic injection technique containing virulent Mycobacterium tuberculosis (H37Rv). After 30 days, the brain and lung CFU were analyzed and histology. Results: In this study, we observed large numbers of bacilli in the CNS determined by the CFU. Moreover, histological analysis showed an intense inflammatory activity in the ventricle while the animal was challenged with H37Rv. Thus demonstrating a model infection with H37Rv strain infused into the lateral ventricle of the CNS in male C57BL / 6 mice. In conclusion, this model is the closest to the normal course of disease in humans, as presented granulomatous tissue lesion characteristics therefore an important strategy for studying the pathophysiology well as mediators involved in the progression, and prevention of this disease.