Avaliação citológica em base líquida de fármacos moduladores estrogênicos

• Ano de defesa: 2006
• Autor(a) principal: Martins, Rand Randall
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Rio Grande do Norte
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Farmacêuticas
• Departamento: Bioanálises e Medicamentos
• País: BR
• Palavras-chave em Português: Inibidores da aromatase; Citologia em base líquida; Modelo animal
• Palavras-chave em Inglês: Aromatase inhibitors; Liquid based cytology; Animal model
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::FARMACIA::FARMACOTECNIA
• Link de acesso: https://repositorio.ufrn.br/jspui/handle/123456789/13511

Hormone therapy is an important tool in the treatment of breast cancer and tamoxifen represents one of the most important drugs used in this type of treatment. Recently other drugs based on the inhibition of aromatase had been developed, this enzyme is responsible for the synthesis of estrogenic esteroids from the androgenic ones. The objective of this study would be the development of a quantitative cytological model of murine estral analysis that allowed the characterization of different hormone drugs effect over vaginal epithelium. The technique of monochromatic staining with Evans blue (C.I. 23860) showed to be efficient in the qualitative and quantitative classification of the cycle. It had been observed differences in the cytological standard of animals submitted to the studied drugs; tamoxifen presented a widening of phases of lesser maturation (diestrais), while anastrozole and exemestane increased the duration of the phases of larger maturation (estrais). The data were analysed through a cubical non linear regression (spline) which allowed a better characterization of the drugs, suggesting a proper cytological profile to the antagonism of the estrogen receptor (tamoxifen), aromatase competition (anastrozole) and inhibition of the enzyme (exemestane)