Avaliação da expressão da fibronectina e tenascina após capeamento pulpar utilizando diferentes agentes hemostáticos (modelo humano) e diferentes materiais capeadores (modelo animal)

• Ano de defesa: 2006
• Autor(a) principal: Baldissera, Elaine de Fátima Zanchin
• Orientador(a): Tarquino, Sandra Beatriz Chaves
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Pelotas
• Programa de Pós-Graduação: Programa de Pós-Graduação em Odontologia
• Departamento: Odontologia
• País: BR
• Palavras-chave em Português: Biocompatibiblidade; Micronúcleos; Imunoistoquimica; Hipoclorito de sódio; Clorexidina; Fibronectina; Tenascina; Matriz extracelular; Dentística; Capeamento pulpar Fibronectina; Agentes hemostáticos
• Palavras-chave em Inglês: Pulp repair; Pulp-capping; Haemostatic agents; Immunohistochemistry; Tenascin; Fibronectin
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: http://guaiaca.ufpel.edu.br/handle/123456789/2300

Aim Based on the great importance of the extracellular matrix (EM) in tissue development and repair, the aim of this study was to investigate the expression of their major glycoproteins, tenascin (TN) and fibronectin(FN) in the human pulp repair. Methodology Using immunohistochemistry, the expression of TN and FN was analyzed in forty-two human teeth, which were taken from a previous study. TN and FN profiles were evaluated after 7, 30, and 90 days after pulp capping with calcium hydroxide, being used three different haemostatic agents (0.9% salin solution, 5.25% sodium hypoclorite and 2% chlorhexidine digluconate) before pulp capping. Results There was no difference in the expression of TN and FN among the distinct haemostatic agents being all the time intervals taken into consideration. Both glycoproteins were found in all the pulp tissue, accomplishing the collagen fiber, and they were absent in all the mineralized tissues. Within 7 days post-treatment, it was observed a slightly more pronounced immunostaining on the exposure pulp site. Within 30 days, TN and FN demonstrated a stronger expression around the dentin barrier. TN also showed focal staining inside the reparative dentin, which was in mineralization. Within 90 days, it was observed a thin and linear expression of TN and FN delimitating the reparative dentin. In the predentin, TN showed strong immunostaining, and FN had a variable expression. Conclusions Based on the results, it may be concluded that there was no difference in the expression of TN and FN among the distinct haemostatic agents being all the time intervals taken into account. Moreover, TN and FN were present in pulp repair,confirming their active participation in this event. It might also be suggested that both glycoproteins are responsible for the odontoblastic differentiation and for the maintenance of the cell shape. It can also be concluded that TN and FN are important factors in the mineralization of the newly-formed dentin matrix.