Avaliação da atividade anti-inflamatória e ensaios toxicológicos não clínicos do extrato etanólico bruto das folhas da Wissadula periplocifolia (L.) C. PRESL

• Ano de defesa: 2016
• Autor(a) principal: Guedes, Edla Julinda Ribeiro Coutinho Espínola
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Farmacologia; Programa de Pós-Graduação em Desenvolvimento e Inovação Tecnológica em Medicamentos; UFPB
• Palavras-chave em Português: Toxicidade; Wissadula periplocifolia; Rato wistar; Anti-inflamatório; Toxicity; Wistar rat; Anti-inflammatory; CNPQ::CIENCIAS DA SAUDE::FARMACIA
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/123456789/11238

Introduction: the species Wissadula periplocifolia (l.) c. Presl., the Malvaceae family, known as river Rafter or malva-Mallow, has popular use as anti-inflammatory, antimicrobial, in addition to reports of use against insect bites and snakes. Objective: to Investigate the potential anti-inflammatory as well as perform the non-Clinical Toxicology Study acute and chronic with crude ethanolic extract of leaves of w. periplocifolia, according to a guide for the conduct of non-clinical studies of Toxicology and safety pharmacology necessary to develop medicines from ANVISA, 2013. Methodology: For evaluation of anti-inflammatory activity was held the paw edema, with Wistar rats receiving (v.o.), vehicle (0.9% saline), antiinflammatory steroidal default not indomethacin and 3 different doses of BSE of w. periplocifolia (25, 50 and 100 mg/kg). Soon after it was measured the volume of the animal's paw (t = 0). After 60 ' was induced the inflammatory process by the administration of 0.1 µ L of the 1% carrageenan subplantar region posterior right foot of each animal. The volume of the Paw was measured immediately after cg injection. and in 4 hours with breaks of 60 ' with the aid of a pletismômetro. In acute toxicity were used, n = 6 Wistar rats of both sexes, with a dose of 2000 mg/kg, orally, of the BSE administered to a treated group and a vehicle control group. After administration, behavioral parameters were observed for 30 ', 60 ', 90 ', 120 ', ' 180 and 240 min., water consumption and haematological and biochemical parameters, feed; and occurrence of death. Already in chronic toxicity the substance was administered daily for a period of 90 days in 6 groups with n = 10. Three groups were treated daily with 3 doses of BSE, of 10, 30, 90 mg/kg. The fourth group, control, water was administered, vehicle used in the dissolution of the BSE. The fifth and sixth groups (satellites) received doses of 30 and 90 mg/kg. We evaluated the effects of prolonged administration as literature. Results: the results of this study demonstrated that 25 and 50 mg/kg of BSE produced anti-inflammatory activity comparable to that of indomethacin, although not dose-dependent. No death occurred with a dose of 2,000 mg/kg, indicating low toxicity. The reduction in feed intake in the Group of males treated and creatinine reduction observed in this group indicated there is loss of muscle mass. There were no changes in haematological parameters. In the chronic study the BSE, promoted a significant decrease of the white count and platelet series, only in males treated groups. There was also a hematocrit decrease of both the satellite and the females males with increased MCHC, showing erythrocyte toxicity, which would need further study. Conclusion: this study previously unreleased non-clinical pharmacological research in Wistar rats, showed potent anti-inflammatory activity not dose dependent. In the acute toxicity study the DL was higher than the 2000 mg/kg, demonstrating there is low toxicity and chronic toxicity study, demonstrated the occurrence of significant changes, which require further studies.