Treinamento de força e suplementação alimentar com Spirulina platensis modulam a reatividade vascular da aorta de ratos wistar saudáveis dependente do no e da atividade antioxidante

• Ano de defesa: 2014
• Autor(a) principal: Brito, Aline de Freitas
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraí­ba; BR; Farmacologia; Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos; UFPB
• Palavras-chave em Português: Exercício; Vasorrelaxamento; Vasoconstricção; Óxido nítrico; Atividade antioxidante; Exercise; Vasorelaxation; Vasoconstriction; Nitric oxide; Antioxidant activity; CIENCIAS BIOLOGICAS::FARMACOLOGIA
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/tede/6838

The species Spirulina platensis has been linked to improved vascular function, but the effect of its association with strength training on vascular reactivity, had not yet been investigated. The objective was to verify the effects of strength training and S. platensis on vascular reactivity, biochemical parameters and oxidative stress in aorta of Wistar rats. The animals were divided into eight groups: sedentary (SG) and supplemented with S. platensis at doses of 50 (SG50); 150 (SG150) and 500 (SG500) mg/kg and trained groups (TG) and supplemented with S. platensis at doses of 50 (TG50); 150 (TG150) and 500 (TG500) mg/kg. The TG performed strength training for 8 weeks. Biochemical analysis was used to quantify the activity of creatine kinase (CK) and lactate dehydrogenase (LDH) levels of reactive C protein (RCP), nitrite, malondialdehyde (MDA) and antioxidant activity. To evaluate the vascular response induced is a cumulative concentration-response curve to phenylephrine (PHE) (10-9-10-3 M) and acetylcholine (ACh) (10-11-10-4 M). To assess the involvement of nitric oxide used is L-NAME and then a cumulative concentration-response curve to the FEN (10-11-10-3 M) was induced. It has been shown so training reduced by around 30% of time jumps without increasing the activity of LDH, CK, CRP and MDA in any of TG when compared to its control group. The functional experiments, it was observed that for contractile reactivity between the SG groups, only SG500 (pD2 = 5.6 ± 0.04 vs. 6.1 ± 0.06, 6.2 ± 0.02, and 6.2 ± 0.04), a significant reduction in the contractile power to FEN. However GT150 and GT500 for groups reduced the contractile power to FEN (pD2 = 5.0 ± 0.06 and 5.3 ± 0.05 vs. 5.6 ± 0.07 and 5.5 ± 0.05). Being that a dose of 500 mg/kg in the trained group reduced the contractile power above the sedentary. It was also found that the ability of strength training and S. platensis in reducing the contractile response was eliminated in the absence of the endothelium and the presence of L-NAME provided greater contractile response to FEN in all groups, and this enhanced response in TG and SG groups. Regarding the relaxing activity in the SG150 groups (pD2 = 7.0 ± 0.08 vs. 6.4 ± 0.06) and SG500 (pD2 = 7.3 ± 0.02 vs. 6.4 ± 0.06) the curve was shifted to the left, similarly the TG150 groups (pD2 = 7.6 ± 0.08 vs. 7.3 ± 0.02) and TG500 (pD2 = 8.0 ± 0.04 vs. 7.3 ± 0.02) also showed the same behavior. However, supplementation at doses of 150 and 500 mg/kg with training further increased relaxant response to ACh. The role of NO was increased when there was significant increase in nitrite production in the aorta of TG150 and TG500. The production of MDA was reduced in all groups around 80%, while the oxidation inhibition percentage was increased by around 50%, mainly from the TG and SG at doses of 150 and 500 mg/kg indicating an enhancement in action antioxidant between such an association. Thus, the present study demonstrated that chronic supplementation of S. platensis at doses of 150 mg/kg and 500 mg/kg or a strength training program alone are effective in promoting an increase in reactivity to ACh relaxing and a decrease in reactivity FEN contractile in the rat aorta, and that the combination of these two procedures enhances the effects. These effects are accompanied by an increase in nitrite concentration, indicating involvement of the NO pathway, by a reduction of oxidative stress and increased antioxidant activity.