Hibridização do esmalte dentário com resina fluida usando o fluxo eletrocinético

Detalhes bibliográficos
Ano de defesa: 2014
Autor(a) principal: Macena, Marcus Setally Azevedo
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal da Paraíba
Brasil
Odontologia
Programa de Pós-Graduação em Odontologia
UFPB
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: https://repositorio.ufpb.br/jspui/handle/tede/8877
Resumo: The transport of materials trough dental enamel has been regarded as dependent on diffusion which doesn´t infiltrate the nanopores of normal dental enamel normal, being these materials considered as above the threshold for enamel nanopores (MATEN). In this in vitro study, applied the electrokinetic flow (EKF) to infiltrated the MATEN into the dental enamel pores mature of unerupted permanent human teeth. It were tested an aqueous solutions with refractive index of 1.56 (Thoulet’s solution) by 5h and a infiltrant resin ICON® by three different times (1; 1,5 and 2 h), both with pH higher than the critical pH of enamel, applying 5V in both cases. Dental enamel ground sections were mounted on customized microfluidic chips made of poly-methyl-siloxane and submitted to EKF. A pre-infiltration with 1 mM KCl solution by 2 h and 1V. Three samples were infiltrated by 1.56 Thoulet´s solution and eight samples were infiltrated using infiltrant resin. Histological sites (n = 30) in Thoulet´s group were analyzed for water birefringence before and after infiltrated. The sample of resin group were subsequently exposed to in vitro demineralization by 192h. The depth of hybrid layer was analyzed by fluorescence and polarizing microscopy. The analysis of mineral volume by radiomicrography was performed in infiltrated histological sites (n = 30) and not infiltrated histological sites (n = 30), before the infiltrated and after demineralization, always hydrated. The negative birefringence of histological points (n = 30) in water, before infiltration of Thoulet’s solution (mean of -22.47 x 10-4) was significantly lower than after the infiltrated of Thoulet’s solution 1.56 (mean of – 51.7 x 10-4) (p < 0.00001; ANOVA; effect size = 6.69). Depth of enamel hybrid layer ranged for 640 m to 1430 m (mean of 760 m). The hydrated minerals volumes of infiltrated parts before demineralization were similar to after demineralization (p = 0.123; T test) while the not infiltrated parts presented similar hydrated mineral volumes after demineralization (p < 0.00001; effect size = 3.263). It is concluded that EKF was successful in infiltrating MATEN in enamel pores, without enamel etching, resulting in a new hybrid layer model and a huge increase in infiltration compared to diffusion, opening new opportunities for scientific innovation and with potential clinical applications in Dentistry.