Avaliação do perfil proteico e atividade biológica do soro de colostro caprino

• Ano de defesa: 2017
• Autor(a) principal: Araújo, Francielly Negreiros de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Biologia Celular e Molecular; Programa de Pós-Graduação em Biologia Celular e Molecular; UFPB
• Palavras-chave em Português: Soro do colostro; Atividade antioxidante; Atividade antibacteriana; Colostrum serum; Antioxidant activity; Antibacterial activity; CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/tede/9392

Colostrum from the first secretions of the mammary glands is considered an essential food for the development of newborns due to its high content of proteins, antimicrobial peptides, macro and micronutrients. The serum is considered na important coproduct for the dairy industry, configuring about 85-90% of the total milk volume, its proteins are recognized by their biological properties, since they have an excellent composition and bioavailability of essential amino acids. The present study had as objective to characterize the serum protein profile of goat colostrum produced by goats Created in Paraíba and to evaluate possible biological activities. The serum (SR) was obtained by acid precipitation in pH 4.1 and fractionated by ammonium sulfate precipitation to obtain the fractions 0-30% (SF1), 30- 60% (SF2) and 60-90% (SF3). The analysis of the protein profile was verified by SDS-PAGE and total amino acids. The biological properties of the samples were verified with the analysis of antioxidant, DNA protection and antibacterial activities. It was verified that colostrum serum presents proteins with molecular masses between 12-160kDa and an excellent profile of amino acids. From the obtained results it is possible to affirm that all the fractions were able to sequester the radicals ABTS and DPPH. For the DNA protection test, the concentracion of 10μg of SF3 conferred a greater integrity to the molecule. The samples presented inhibitory activity for both gram-negative and gram-positive bacteria. Colostrum serum proteins have biological properties with potential biotechnological application and pharmaceutical interest