Detalhes bibliográficos
| Ano de defesa: |
2022 |
| Autor(a) principal: |
Bandeira, Thuany Lura Teixeira |
| Orientador(a): |
Ourique, Aline Ferreira |
| Banca de defesa: |
Bonadiman, Beatriz da Silva Rosa,
Dalla Nora , Lissandro Dorneles |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso embargado |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Franciscana
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Nanociências
|
| Departamento: |
Biociências e Nanomateriais
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1125
|
Resumo: |
Açaí oil is a by-product of the native fruit of the Amazon region that has proven to have excellent properties such as: antimicrobial, anti-inflammatory, prevention and combat of cardiovascular diseases, neurodegenerative neurological diseases, but this oil despite recognized beneficial properties to humans, it has limitations such as low aqueous solubility and easy susceptibility to degradation, characteristics that can compromise its performance. However, nanostructuring guarantees greater stability in terms of oxidation and other environmental effects that may compromise the effectiveness of the product. Thus, the objective of this work is to prepare, characterize nanoemulsions containing açaí oil, and evaluate the in vitro safety profile and the potential neuroprotective effect on microglia via modulation of rotenone-induced mitochondrial dysfunction. For this, initially the characterization and solubility of açaí oil were carried out, then the preparations of nanoemulsions were carried out, through two methods, namely: spontaneous emulsification and high agitation emulsification. The nanoemulsions were characterized in terms of average droplet size, polydispersity index, zeta potential, pH, morphological analysis and centrifugation test of the nanoemulsions. After the characterizations, the evaluation of the free radical scavenging activity was carried out using the DPPH and ABTS methods. Then, in vitro treatments were performed based on a concentration-effect curve (0.001 – 1000 µg/mL) of the nanoemulsions in normal fibroblasts of the HFF-1 lineage and an efficacy profile in reversing mitochondrial dysfunction in microglial cells of the BV lineage. -two. The açaí oil was solubilized using surfactants. The nanoemulsions prepared using two methods (top-down and bottom-up strategies) showed a droplet size of approximately 94nm and 175nm, low polydispersity index, Zeta potential of -7.4 mV and -16.03 mV, pH 5.9 and 5.8 and stability of up to 90 days at room temperature and under refrigeration. The morphological analysis of the nanoemulsions corroborated the results obtained by dynamic light scattering and demonstrated spherical droplets. Nanoemulsions and açaí oil showed low antioxidant activity. While the results of the in vitro assays showed that most concentrations were safe, only the highest concentrations were cytotoxic and genotoxic to cells, showing a neuroprotective effect in an in vitro model, in both emulsification methods, but not reverse mitochondrial dysfunction. These results indicate a perspective of therapeutic use for the developed nanoemulsions. |
