Detalhes bibliográficos
| Ano de defesa: |
2024 |
| Autor(a) principal: |
Montagner, Giane Engel |
| Orientador(a): |
Gomes, Patrícia |
| Banca de defesa: |
Carvalho, José Antônio Mainardi de,
Colet, Christiane de Fatima,
Martins, Mirkos Ortiz,
Rhoden, Cristiano Rodrigo Bohn |
| Tipo de documento: |
Tese
|
| Tipo de acesso: |
Acesso embargado |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Franciscana
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Nanociências
|
| Departamento: |
Biociências e Nanomateriais
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1319
|
Resumo: |
SARS-CoV-2 infection triggers an excessive inflammatory process, causing hematological changes. In this context, antiviral and anti-inflammatory treatments are essential. Phenolic compounds found in grape seed and guarana are promising for this treatment, as they are natural compounds with low toxicity. Thus, the aim of this study was to investigate the correlation of laboratory clinical parameters with the progression of COVID-19, to develop a diagnostic and prognostic prediction system, as well as to produce liposomes containing GSE and guarana that can reduce the inflammatory response, along with the drying of these liposomes and their ecotoxicological evaluations. To this end, the correlation of blood tests with the presence and severity of the disease was assessed. Machine learning models were trained to predict diagnosis and prognosis. The compounds caffeine, catechin, epicatechin, procyanidins B1, B2, B3, B4, and B5, theobromine, and theophylline present in grape seed and guarana were tested via molecular docking to evaluate their potential to bind to the SARS-CoV-2 spike protein and ACE-2, preventing infection. Toxicity prediction was assessed using online software. The safety and efficacy of guarana, GSE, and liposomes were tested in lung cell lines. Liposome drying was optimized through experimental design. Ecotoxicity was evaluated using A. salina and Allium cepa tests. Lymphocyte, monocyte, platelet, CRP, and ferritin values were significantly altered in COVID-19-positive patients compared to negative patients. RDW, leukocyte, neutrophil, NLR, SII, SIRI, AISI, and CRP levels were significantly increased in critical patients, and most critical patients had diabetes mellitus, systemic arterial hypertension, and oximetry saturation below 95%. The Naive Bayes model was used to predict COVID-19 diagnosis, correctly identifying 82.8% of patients. The Neural Network model was used to predict patient prognosis, correctly identifying 85.7%. Through online prediction, the compounds showed low toxicity and were able to bind to the spike protein and ACE-2. Guarana, GSE, and their liposomes showed no cytotoxicity at concentrations below 10 μg mL-1, maintaining cell viability without producing reactive oxygen species or causing cellular damage, and they demonstrated anti-inflammatory activity. The optimal drying condition for the liposomes was 112 ºC, 4.39 m3 min-1, 0.6 L h-1, and 6.5% maltodextrin. GSE, guarana, and the liposomes were considered to have low toxicity for A. salina and Allium cepa. |
