Detalhes bibliográficos
| Ano de defesa: |
2021 |
| Autor(a) principal: |
Druzian, Daniel Moro |
| Orientador(a): |
Silva, William Leonardo da |
| Banca de defesa: |
Volkmer, Tiago Moreno,
Ourique, Aline Ferreira |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Franciscana
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Nanociências
|
| Departamento: |
Biociências e Nanomateriais
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1013
|
Resumo: |
Due to the increased life expectancy of the population has resulted in an increase in inflammatory degenerative bone problems, leading to problems of prolonged pain and physical. Thus, biomaterials emerge as an alternative potential for partial or complete replacement of bone structures, especially the nanobioglass as it is a synthetic material with osteoconduction and resorption properties. Thus, this work aims to synthesize and characterize a nanobioglasses of the SiO2-P2O5-CaO-MgO system, using rice husk residue as a silica precursor in order to improve the osteoconductivity of the white nanobioglass for bone tissue applications. For the synthesis of nanobioglasses, sol-gel process was used, followed by calcination (873 K and 2 hours) using silica extracted from rice husk and magnesium oxide nanoparticles synthesized by thermal methods. Moreover, experimental planning was carried out by the Central Composite Rotational Design (CCRD 2²) in order to determine the ideal condition for the synthesis of nanobioglass, evaluating the pH and time parameters. Thus, silica oxide nanoparticles (SiNPs), magnesium oxide nanoparticles (MgNPs) and nanobioglasses (NPs -BV and MgNPs-BV) were characterized using elementary, morphological, structural and textural techniques by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy with Energy Dispersion Spectroscopy (SEM-EDS), N2 porosimetry (BET/BJH method) and zeta potential (ZP). As for the biological analysis, the cytotoxicity effect was verified using HFF-1 fibroblasts through MTT (cellular viability), NO (nitric oxide), dsDNA (fluorimetric quantification of dsDNA) and DCFHA (evaluation of the total rate of reactive species assays oxygen). Regarding the CCRD 2² experimental design for the NPs-BV process, the ideal condition was t = 20 min and pH = 8, which was chosen to carry out the other characterization tests. SiNPs showed an amorphous phase without the presence of impurity peaks, with a spherical morphology (average size of 40-50 nm), specific surface area (SBET) of 26.25 (m2.g-1) and elemental composition of silicon (55.27%), and oxygen (44.73%), confirming the achievement of SiNPs. About the MgNPs, showed a crystalline phase (periclase), with an average crystallite diameter of 21.1 nm and 99.8% of crystallinity, specific surface area (SBET) of 16.79 (m2.g-1), regarding the elemental composition. oxygen (51.15%) and magnesium (48.85%), confirming the achievement of MgNPs. NPs-BV and MgNPs-BV showed amorphous characteristics. Furthermore, doping with MgNPs promoted an increase of about 3 times in the surface area of the nanobioglass (101.33 to 334 m².g-1), an increase of 56% and in the pore volume. The elemental analysis showed characteristics of a 40S nanobioglass, with the presence of phosphorus ions, confirmed by FTIR. Regarding biological tests, the extracts of NPs-BV and MgNPs-BV showed a high safety profile of cytotoxicity, biocompatibility and bioactivity, highlighting the extract of 100% MgNPs-BV, as it presented twice the cell proliferation, when compared to the negative control. Therefore, nanobioglass prepared from rice husk and containing magnesium nanoparticles has potential application as a scaffold in processes involving bone regeneration, and can be considered a promising biomaterial to be developed. |
