Efeito do resveratrol complexado à hidroxipropil-β-ciclodextrina sobre parâmetros do estresse oxidativo em ratos normais e hiperglicêmicos

• Ano de defesa: 2014
• Autor(a) principal: Pinto, Claudia Grigolo
• Orientador(a): Fernandes, Liana da Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Franciscana
• Programa de Pós-Graduação: Mestrado Acadêmico em Nanociências
• Departamento: Biociências e Nanomateriais
• País: BR
• Palavras-chave em Português: Nanotecnologia; ciclodextrinas; resveratrol; estresse oxidativo
• Palavras-chave em Inglês: Nanotechnology; ciclodextrins; resveratrol; oxidative stress
• Área do conhecimento CNPq: CNPQ::ENGENHARIAS
• Link de acesso: http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/229; http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/309

The use of nanoparticles as carriers of drugs has been widely studied. These include cyclodextrins (CDs), cyclic oligosaccharides that allow the formation of inclusion complexes with lipohilic substances such as resveratrol (RSV). RSV is a polyphenol with antioxidant characteristics; when consumed, a great part is excreted, while the remainder is quickly metabolized in the body. The anti-inflammatory, antioxidant and preventive of metabolic diseased properties such as diabetes, stimulate the complexation of RSV to the hydroxypropyl-beta-cyclodextrin (HP-β-CD), thus enabling the increase of the bioavailability of an orally RSV. Hyperglycemia, a marker by which diabetes is diagnosed, promotes increased production of species reactive to oxygen by various routes that lead to cell damage and potentially to cell death. The oxidative stress parameters can be determined through certain techniques. The reduction in the amount or in the activity of endogenous antioxidant defenses is and indicative of oxidant injury and may, therefore, is considered as an excellent marker of oxidative stress. In this context, nanotechnology can contribute to the development of new therapeutic approaches for various diseases, including diabetes. The objective of this study is to investigate the in vivo effects of free and complex resveratrol to HP-β-CD on parameters of oxidative stress, based on the measurements of GSH, TBARS, total content of carbonyls, activity of SOD, CAT and GPx enzymes and determination of total protein in the cerebral cortex, liver, kidney and heart of normal and hyperglycemic rats. For this study, 70 male Wistar rats were used, form the vivarium of the Federal University of Santa Maria, randomly divided into five diabetic groups induced by streptozotocin (STZ), and five non-diabetic groups with seven animals per group. The hyperglycemic and normoglycemic groups daily received, through intragastric gavage, the following substances: water (control groups), free RSV in concentration of 1mg/kg (RSV groups), concentration of HP- β-CD equivalent to that present in complex (HP- β-CD groups), RSV complexed to the cyclodextrin at a concentration of 1 mg/kg body weight (complex groups) and a solution of 15% ethanol (ethanol group). After two months of treatment and 12 hours fasting, the animals were killed by decapitation. According to the data obtained, it was found that treatment with HP- β-CD caused a decrease of endogenous antioxidant molecules. When complexed to RSV, this decrease was lower; indicating a protective effect by RSV to the damage caused by the medium, in this case HP- β-CD. In the measurements of TBARS it was observed that there was a decrease in the RSV group and HP- β-CD, indicating a protective behavior to lipid peroxidation.