Análise de flavonóides por cromatografia líquida de alta eficiência (CLAE), com ensaios in vitro do potencial antioxidante em folhas de Smilax fluminensis Steud. (Smilacacea)

• Ano de defesa: 2012
• Autor(a) principal: Petrica, Edith Eunice Arthur
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Instituto de Ciências Exatas e da Terra (ICET); UFMT CUC - Cuiabá; Programa de Pós-Graduação em Química
• Palavras-chave em Português: Flavonóides; Cipó japecanga; Salsaparrilha; Potencial antioxidante; CLAE; RMN; Smilax Fluminensis Steud; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA; Flavonoids; Japecanga; Sarsaparilla vine; Antioxidant potential; HPLC; NMR
• Link de acesso: http://ri.ufmt.br/handle/1/1641

The objective of this study was to identify and quantify flavonoids present in the ethanol extract of leaves of Smilax fluminensis by high performance liquid chromatography (HPLC-UV), to evaluate the antioxidant potential of the fractions, isolate and identify some flavonoids. Were identified and quantified by HPLC, eight flavonoids: agatisflavone, amentoflavone, apigenin, kaempferol, quercetin, quercitrin, rutin and taxifolin. The content of flavonoids identified by HPLC ranged from 2.0 ppm to 125 ppm. The antioxidant potential was shown by the fractions above 90% of DPPH sequestration in most fractions with EC50 4.0 mg/mL to 14.0 mg/mL. Have been isolated and identified flavonoids: Quercetin[3-O- -L-ramnopyranosyl(1’’’ 6’’)- -D-glucopyranosyl]-7-O- - L-ramnopyranoside (SG116-124); quercetin-3-O-b-D-galactopyranoside (hiperin) (SG125-161); Quercetin-3-O- -L-ramnopyranosyl(1’’’ 6’’)-O- - D-glucopyranoside, (SG201-215) and quercetin (SH29-32). The HPLC method allowed the identification and quantification of flavonoids, supported by analysis of Nuclear Magnetic Resonance - NMR 1H and 13C and mass spectrometry.