Detecção de diferentes genótipos de rotavírus tipo A em um surto de diarreia neonatal em bovinos de corte no estado de Mato Grosso

• Ano de defesa: 2017
• Autor(a) principal: Rondelli, André Luiz Hoeppner
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Faculdade de Medicina Veterinária (FAVET); UFMT CUC - Cuiabá; Programa de Pós-Graduação em Ciências Veterinárias
• Palavras-chave em Português: Bezerros; Doença entérica; Rotavírus; Lesões macroscópicas; Genótipos; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA; Calves; Enteric disease; Rotavirus; Gross lesion; Genotypes
• Link de acesso: http://ri.ufmt.br/handle/1/3724

Calf diarrhea causes substantial economic losses to beef cattle production worldwide. It is a complex multifactorial pathological condition influenced by infectious, nutritional and environmental factors. The present study focused on analyzing the pathological and molecular characterization of bovine rotavirus A (BoRVA) during a diarrhea outbreak in a beef cattle herd located in the state of Mato Grosso, central-western region, Brazil. The outbreak caused high morbidity (80%) and lethality (12%) among 1,100 calves up to 30 days of age. The BoRVA was identified in 53.3% (16/30) of the diarrheic fecal samples analyzed using the silverstained polyacrylamide gel electrophoresis (ss-PAGE) technique. The nucleotide sequence analysis of VP7 (G genotype) and VP4 (P genotype) via RT-PCR from eight BoRVA-positive fecal samples showed the genotypes G6P[5] (n = 6), G6P[11] (n = 1) and G6P[X] (n = 1). Three calves were necropsied and the gross findings included edema and thickened, wrinkled bowel mucosa in the small intestine. Microscopic lesions were confined to the villi of the small intestine, characterized mainly by atrophy and non-suppurative inflammatory lesions. Immunohistochemical examination of three cases was positive for BoRVA. The 53.3% of the diarrheic fecal samples that were positive for BoRVA in this study suggested that RV was the etiological agent involved in this neonatal calf diarrhea outbreak. Rotavirus shows genomic variability and complexity, and only through molecular characterization of BoRVA strains circulating in cattle herds can the most frequent genotypes be determined and help improving vaccines for calf diarrhea and implement efficient control and prophylaxis program.