Efeitos do Paraquat sobre os comportamentos do tipo ansiedade e medo no Zebrafish (Danio rerio)

• Ano de defesa: 2020
• Autor(a) principal: Martini, Árthur Galvão
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Instituto de Educação (IE); UFMT CUC - Cuiabá; Programa de Pós-Graduação em Psicologia
• Palavras-chave em Português: Zebrafish; Ansiedade; Medo; Morfologia encefálica; Paraquat; CNPQ::CIENCIAS HUMANAS::PSICOLOGIA; Anxiety; Fear; Brain morphology
• Link de acesso: http://ri.ufmt.br/handle/1/6122

The project aimed to verify the effects of three different doses of Paraquat (5mg/Kg; 10mg/Kg and 20mg/Kg) on anxiety and fear morphological and behavioral changes in Zebrafish. 168 animals were tested (n = 168) divided into 5 groups, control group without saline, control group with saline, experimental group under-dose, experimental group with standard dose and experimental group with over-dose. The different animals were exposed to three behavioral tests: a) light / dark test, novel tank test and alarm substance evoked anxiety test and morphological analysis of the brain. The results indicated that Paraquat produced dosedependent effects on anxiety and fear behaviors, highlighting that, in the over-dose group (20mg / Kg), nerve cell involvement was observed, causing changes in self-preservation behaviors. In the sub-dose (5mg / kg) and standard dose (10mg / kg) groups, there was an increase in anxiety and fear behaviors indicating a greater sensitivity to aversive stimuli present in the environment. Thus, it can be concluded that Paraquat alters the morphological organization of the neuronal cell, as well as, some anxiety and fear behaviors in Zebrafish.