Avaliação das atividades antimicrobiana e imunomoduladora e quantificação de marcadores vegetais em extratos secos de Echinacea Purpurea

• Ano de defesa: 2018
• Autor(a) principal: Barbara Goncalves de Oliveira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Ácido caftárico; Echinacea purpurea; Ácido chicórico; Atividade imunomoduladora; CLUE-UV; Ácido Clorogênico; Infecções; Cromatografia Líquida de Ultra Eficiência (CLUE); Atividade antifúngica; Plantas medicinais; Ácidos
• Link de acesso: http://hdl.handle.net/1843/BUOS-BAVJRA

Medicinal plants are widely used for therapeutic purposes in several pathologies that affect human health. With regard to Echinacea purpurea (EP), a plant of North American origin, its use is widely diffused throughout the world, presenting wide pharmacological application, mainly in the treatment of infections of respiratory and urinary tracts. In Brazil, dry extracts of EP are used to the production of medicines by compounding pharmacies and pharmaceutical industries, however, the source of them is not always well established. So, it is extremely important to evaluate the quality and efficacy of these extracts for the treatment of infections. Therefore, the objective of this work was to develop and validate an analytical method by ultra performance liquid chromatography coupled to a diode array detector (UPLC-DAD), to identify and quantify the chemical markers in EP. UPLC method was developed using a C18 (2.1 x 50 mm, 1.8 m) chromatographic column, maintained at 30 °C. The mobile phase was composed of acetonitrile and 0.05% (v/v) formic acid aqueous solution, in a ratio of 9:1, in isocratic mode, flow rate 0.2 mL/min. The injection volume was 10 L and the wavelength for detection was 300 nm. The developed method complied with the required validation parameters, and showed to be linear, precise, accurate, selective and robust. Using the validated method, the levels of caftaric acid, chicoric acid and chlorogenic acid were quantified in five samples of EP dry extracts obtained, with a significant variation in the contents between the different samples. In vitro biological tests were also performed to evaluate the antimicrobial and immunomodulatory activities of dry extracts of EP. The results showed that the analyzed samples showed no significant antibacterial activity, but a low antifungal activity was verified. Positive effects of Echinacea purpurea extracts on the modulation of cellular immune activity were also observed.