Campilobacteriose genital bovina na sub-região da Nhecolândia do Pantanal Sul-Mato-Grossence e proposição de novas técnicas de diagnóstico

• Ano de defesa: 2001
• Autor(a) principal: Aiesca Oliveira Pellegrim
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: elisa IgA; reprodução; pantanal; diagnóstico; Campilobacteriose; Infecções por campylobacter Diagnóstico; Bovino Doenças; Campylobacter
• Link de acesso: http://hdl.handle.net/1843/BUOS-8BZMLJ

The estimated prevalence of Bovine Genital Campylobacteriosis the fold of the Pantanal was performed in 19 clusters by direct iminofluorescência washed in preputial yielding 52.3% (170) prevalence in bulls (95% CI, 42.6% - 62.0%) and 89.5% (17) of positive properties. A standardized enzyme immunoassay for detection of immunoglobulin A anti Campylobacter fetus (C. fetus) in cervicovaginal mucus with an antigen extracted with glycine buffer. the measurement of optical density readings (OD450) was 0143 + _ 0.09. By immunoblotting the proteins in the most immunoreactive antigen of the> fetus subsp. Venerealis (C. fetus subsp. Veneralia) and Campylobacter fetus subsp. Fetus (C. fetus subsp. Veneralia) migrated at 45 kDa but the protein of 97kDa was recognized Sell Your by C. fetus subsp. Veneralia. The immunoenzymatic assay was used to detect IgA antibodies against C. fetus in 311 samples of vaginal mucus fêmias from 18 herds in the region of the Pantanal and specificity of ELISA IgA was estimated at 94.4%