Avaliação do possível efeito dual (antioxidante e/ou pró-oxidante) e ação neuroprotetora do ebselen, ácido caféico e memantina em células neurais (Neuro-2A) in vitro

• Ano de defesa: 2012
• Autor(a) principal: Paula Andrade da Fonseca Prado
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Neuro-2A; ROS; Memantina; Ebselen; Estresse Oxidativo; Ácido Caféico; Envelhecimento; Antioxidantes; Neurologia; Neurociências
• Link de acesso: http://hdl.handle.net/1843/BUOS-8TMKEG

Aging is defined by a progressive increase in many deleterious alterations in cells and tissues that enhance the risk of diseases and death. These may be due to changes in the DNA, diminution in protein synthesis, self-attack in the immune system and damage caused by oxidative reactions. Some evidences indicate that aging and chronic/degenerative diseases are primary caused by damage caused by the free radicals, including the reactive oxygen species (ROS). They may attack the most important body molecules. Oxidative stress occurs when ROS generation exceeds the antioxidant capacity of the cell. The aim of this work was to evaluate the possible dual effect (antioxidant and/or prooxidant) and the neuroprotector action of Ebselen, Caffeic Acid and Memantina in neural cells (Neuro-2A) in vitro. ROS generation was quantified by luminal-dependent chemiluminescence. Cell viability was performed by Neutral Red incorporation. The mitochondrial reductor power was made by the MTT assay. Statistical analyses was performed by non-parametric Mann-Whitney, where p<0.05 was considered significant. Our results showed that Ebselen-treated cells in the concentration 5 M had a decrease in ROS production induced by H2O2 and an enhanced mitochondrial reductor power. Caffeic Acid, in all concentrations (25, 50, 100, 200 e 500 M) had an antioxidant effect and an enhanced reductor power except by the highest concentration (500 M). Even though Memantine had an increase in the reductor power in the concentrations 0,5 e 1 M, it was not efficient as an antioxidant. This drug presented a prooxidant effect in the concentration 50 M. In the viability test, none of the substances caused damage in the lysosomal level. Basing on these results, we conclude that the antioxidants studied have anti- and pro- oxidant activities depending on the concentration tested. Therefore, they should be used carefully because they may cause damage to health instead of progress.