Caracterização molecular da microbiota bacteriana da pele lesionada de indivíduo com Hanseníase

• Ano de defesa: 2013
• Autor(a) principal: Paulo Eduardo de Souza da Silva
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Genética; Genetica molecular; Mycobacterium leprae; RNA ribossômico 16S; Genética microbiana; Hanseniase
• Link de acesso: http://hdl.handle.net/1843/BUOS-975JMM

The skin is a critical barrier for the survival by preventing moisture loss and invasion by infectious agents and/or toxic substances. Among the various diseases that can affect the skin, there is leprosy, infectious disease, granulomatous, debilitating, but treatable, caused by Mycobacterium leprae which mainly affects the peripheral nerves. Histopathology is used for the diagnosis of leprosy. Specimens removed by biopsy are fixed and embedded in paraffin, and after performed histological sections, these blocks are filed. These files, classified as "biobanks", are rich sources for research related to biomedical areas. There are few studies on the bacterial diversity of skin lesions of patients with leprosy for both culturable bacteria as mostly unculturable. This study investigated the diversity of bacteria present in histological lesional skin of individual diagnosed with lepromatous leprosy, through a culture -independent approach. Thus, total DNA was extracted from paraffin block. The amplicon of the 16S rRNA gene of bacteria was used for the construction of library clones. Phylogenetic analysis revealed a moderate diversity, but complex, detecting OTUs 27 (operational taxonomic unity) that were affiliated with four phyla: Proteobacteria (48%), Firmicutes (41%), Bacteriodetes (4%) and Actinobacteria (7%). Studies on bacterial diversity of the skin of healthy individuals revealed the predominance of these same phyla, although with different distribution. It is noteworthy that genus Propioniobacterium (Actinobacteria), indigenous bacteria of healthy skin, was drastically reduced in lesional skin of individuals with leprosy. This is the first study of bacterial diversity in lesional skin of individual with leprosy, indicating significant change in the abundance of dominant phyla of normal skin, as well as changes in the composition of indigenous taxa, such as genus. The data obtained are essential to detect differences of bacterial microbiota associated with this disease.