Associação dos sistemas de recombinação em levedura e de genética reversa para a obenteção de rearranjos entre os vírus da influenza humana e equina

• Ano de defesa: 2003
• Autor(a) principal: Aymara Alves Correa Rangel
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: rearranjo genético; Influenza equina; recombinação em levedura; genética reversa; Equino Genética; Clonagem; Influenza eqüina; Vírus da influenza
• Link de acesso: http://hdl.handle.net/1843/BUOS-8C5LHH

The system of recombination in yeast is emerging as a new cloning technology in the middle científicom, being a pioneer in the thesis introduction of this methodology for the cloning of genomic segments of influenza virus. The speed and efficiency of procedures which involve the recombination system in yeast were verified during the production of recombinant plamideos containing the cDNA of the eight genomic segments of the equine influenza virus, A/Equine/Kentucky/2/86 (H3N8). Similarly, these qualities will also guarantee the formation of a collection of plasmids to be used in the production of a viral sample of interest