Perfil protéico do sêmen e fertilidade de touros da raça Gir

• Ano de defesa: 2007
• Autor(a) principal: Isabella de Moura Folhadella
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: parâmetros andrológicos; Cromatografia; eletroforese; Semen Análise; Eletroforese; Gir (Zebu) Reprodução; Fertilização in vitro
• Link de acesso: http://hdl.handle.net/1843/SSLA-7TWGSJ

It was studied semen total protein and heparin binding protein profile of 15 Gyr bulls and itsassociation with andrological characteristics and in vitro fertility potential. It was verified yetassociations among andrological, spermatic and in vitro fertility parameters of nine Gyr bulls.First, animals were submitted to breeding soundness evaluation and andrological classificationby points (ACP) followed by evaluation of total protein profile with gel filtrationchromatography and heparin binding protein of sperm membrane and seminal plasma by FastPerformance Liquid Chromatography (FPLC) with heparin affinity columm. Each peak ofheparin binding protein of semen was submitted to eletrophoresis to identify molecular weight.Protein concentrations identified by LOWRY (1951) and in vitro fertilization was made withoocyte maturation, fertilization and embryo culture. Proteins were associated with in vivoandrological parameters of scrotal circumference, motility, vigor, major and total defects andACP, and with in vitro fertility by cleavage and blastocyst rates. Andrological parameters dateswere associated also with in vitro fertility. Total protein concentration of seminal plasmaranged from 4.1 to 167.9 mg/ml, with mean of 47.1 ± 48.5 mg/ml, with great variability amongbulls. Chromatographic profile of total seminal plasma proteins presented 8 different peaks in100% and 9 peaks in 86% of bulls. Heparin binding proteins presented 5 affinity peaks in87,5% and 3 peaks in 100% of bulls. Greater associations were observed for: total proteins(PLB) X blastocysts rate, peak 1 heparin binding proteins(P1A) X blastocysts rate, peak 5heparin binding protein (P5A) X cleavage rate and peak 5 heparin binding protein (P5A) Xblastocysts rate, and all of them with high magnitude association (>0,66). However none of theproteins showed association with andrological parameters. To the characteristic in vivo motility,regressions were significant (p<0,10) among motility X cleavage and motility X blastocystrates, although with mean magnitude. However few andrological parameters had beensignificant association, it was not possible establish an efficient method for in vitro fertilityprediction based on andrological parameters.