Procedimentos de regeneração pulpar: avaliação longitudinal imune

• Ano de defesa: 2018
• Autor(a) principal: Igor Vieira Bracks
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Regeneração Tecidual Guiada; Vasos Sanguíneos; Reação em Cadeia da Polimerase em Tempo Real; Ácido Etilenodiaminotetracético; Dente Não Vital; Reação em cadeia da polimerase em tempo real; Vasos sanguíneos; Ácido edético; Regeneração tecidual guiada; Dente não vital; Epidemiologia experimental; Endodontia
• Link de acesso: http://hdl.handle.net/1843/ODON-B3XHKV

Pulpar regeneration procedures: Longitudinal imune assessment Endodontic treatment in teeth with incomplete root development is related to some difficulties and limitations. However, despite the technical difficulties, the high susceptibility to fracture of teeth with incomplete rhizogenesis represents the key factor for the search for new therapeutic modalities. Additionally, the aesthetic and functional deficits presented by rehabilitation therapies after the loss of a permanent dental element in a young patient are also important and stimulating factors. Thus, it is evident the need for research that offers new conservative therapeutic options, with predictable results. Aimed to investigate the immunoinflammatory response of teeth submitted to different protocols described in the literature to perform the regenerative endodontic therapy. For this, the expression of inflammatory molecules and cell growth/differentiation factors expressed in pulpal tissues were analyzed at different time intervals using a murine model developed for the present study. 54 Balb/C mice had the pulp chambers of their upper molars opened and subsequently submitted to pulpectomy (one tooth per animal). The animals were then divided into 3 groups: Bleeding group - filling of the pulp space with blood clot; EDTA + Bleeding group (EDTA + Blood) - irrigation of the channels with 17% EDTA solution for 1 min, followed by filling of the pulp space with blood clot; Empty - pulp space left empty (negative control). Each group consisted of 18 animals. From each group, 6 animals were sacrificed at 7, 14 and 21 day intervals after the experiments. Using real-time polymerase chain reaction (RT-PCR), the cytokines IL-1, TNF-, IL-10 and the growth/differentiation factors NGF, IGF and VEGF, comparing such inter and extra group findings in the different evaluation periods. The results showed the highest expressions of the pro-inflammatory mediators in the Empty group, as well as a greater expression of anti-inflammatory mediators in the Experimental group filled with the blood clot. The EDTA + Blood group evidenced the greater gene expression of growth / differentiation factors, in all periods analyzed, when compared to the other groups. It can be concluded that irrigation with 17% EDTA solution, prior to filling the root canals system with the scaffold (blood clot), stimulated the increased expression of detrimental mediators for the success of regenerative endodontic therapy. Additionally, the animal model developed for the research proved to be effective in longitudinally analyzing the immune modulation that occurs in octopus-periradicular tissues after the institution of therapy.