Metabolômica aplicada na pesquisa de biomarcadores para acidente vascular encefálico isquêmico, infarto do miocárdio e esquizofrenia

• Ano de defesa: 2018
• Autor(a) principal: Vânia Aparecida Mendes Goulart
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; Brasil; ICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS; Programa de Pós-Graduação em Biologia Celular; UFMG
• Palavras-chave em Português: Metabolômica; AVE; Infarto; Esquizofrenia; HPLC-MS/MS; Biomarcadores; Acidente vascular cerebral; Espectrometria de massas
• Link de acesso: http://hdl.handle.net/1843/35395

Metabolomics has been outstanding because it is a methodology able to characterize metabolic phenotypes, which are the result of what is expressed by the genome and increased by the environmental influence. Several studies have reported on the effectiveness of this approach for biomarkers research for the diagnosis and prognosis of various diseases. In this study we used high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS / MS) for the analysis of five metabolic classes (amino acids, biogenic amines, phosphatidylcholines, sphingomyelins and hexose) in plasma / serum of patients with ischemic stroke, acute myocardial infarction with ST segment elevation, schizophrenia and controls. For these diseases there are still no biochemical biomarkers for diagnosis or prognosis. In this context, we used a target metabolomic approach and obtained metabolic panels capable of characterizing each of the above mentioned diseases in relation to the controls. According to our results, disorders occur in the glycerophospholipid metabolism due to inflammatory processes in cases of ischemic stroke and acute myocardial infarction, and in the case of patients with schizophrenia, changes in lipid profiles may be related to the use of antipsychotics.