Diversidade e Tolerância de Fungos Endolíticos em Plantas Sujeitas a Estresses Causados por Metais Pesados

Detalhes bibliográficos
Ano de defesa: 2018
Autor(a) principal: SILVA, Leandro Souza lattes
Orientador(a): SIQUEIRA, Glécio Machado lattes
Banca de defesa: SANTOS, Juliano dos lattes, RIBAS, Priscila Pauly lattes, DALL’AGNOLL, Leonardo Teixeira
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal do Maranhão
Programa de Pós-Graduação: PROGRAMA DE PÓS-GRADUAÇÃO EM BIODIVERSIDADE CONSERVAÇÃO/CCBS
Departamento: COORDENAÇÃO DO CURSO DE CIÊNCIAS NATURAIS-BIOLOGIA/CAMPUS III
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: https://tedebc.ufma.br/jspui/handle/tede/5037
Resumo: One of the main problems of the contemporary world is the contamination of soil and water by heavy metals. Many microorganisms, such as fungi, for example, may interact with these heavy metals in the environment acquiring the ability to survive by adaptation or mutations, and are considered promising bioremediation agents. The objectives of this study were: (I) to evaluate the frequency of endophytic fungi in plants of sites subject to contamination by heavy metals, and (II) to evaluate the in vitro tolerance of these fungi to mercury. Leaves of eight plant species were collected along the river banks of the Sabino Tributary Stream, in a region heavily influenced by the Sanitary Landfill of Ribeira, São Luís, Brazil. These leaves were surface disinfested and plated in BDA medium. The recovered endophytic fungi were purified, identified and deposited in the mycological collection of NIBA / UFMA. The mercury tolerance of the 10 predominant species was evaluated by the mycelial growth of these in plates with BDA medium supplemented with HgCl2 in seven concentrations (1 to 7 mg.mL-1). In total, 54 isolates were obtained. Fungi belonged to 28 morphological types distributed in 11 genera. The Tolerance Index (TI) and Mycelial Growth Rate Index analyzes indicated that, despite differences in behavior under stress caused by mercury, many fungi exhibited satisfactory levels of tolerance to the metal. Scytalidium lignicola and Trichoderma sp. showed high tolerance and high growth in media containing high concentrations of HgCl2, indicating the possibility of the use of these fungi in bioremediation essays of contaminated environments.