Caracterização proteômica da fração nuclear da forma leveduriforme de espécies de Paracoccidioides

• Ano de defesa: 2014
• Autor(a) principal: Oliveira, Lucas Nojosa
• Orientador(a): Soares, Célia Maria de Almeida
• Banca de defesa: Soares, Célia Maria de Almeida, Borges, Clayton Luiz, Pereira, Maristela
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Biologia das Interações PH (IPTSP)
• Departamento: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
• País: Brasil
• Palavras-chave em Português: Paracoccidioides; Núcleo; Proteoma
• Palavras-chave em Inglês: Nucleus; Proteoma
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::PARASITOLOGIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/4058

Paracoccidioidomycosis (PCM) is an endemic disease in Latin America caused by fungi of the genus Paracoccidioides which is composed of two species P. lutzii e P. brasiliensis. The fungus grows as mycelium in saprophytic environment, and as yeast in host. Paracoccidioides spp present multinucleated yeast cells. The characterization of the nuclear proteome an important step to understand the biology of the fungus, and contributes to future studies of possible pharmacological targets. In this study we aimed to characterize the profile of the nuclear proteome of Paracoccidioides species in yeast form. A total of 570 proteins were identified and classified as nuclear, using a combination of method sample enrichment, proteomic of high accuracy technique, NanoUPLC-MS E , and bioinformatic filters. The results revealed important proteins related to DNA maintenance, regulation and gene expression, synthesis and processing of messenger and ribosomal RNA and nuclear-cytoplasmic traffic. Uncharacterized proteins and proteins commonly known as cytoplasmic were identified in fraction enriched nuclear and require further studies to demonstrate its nuclear roles. This is the first descriptive nuclear proteome of Paracoccidioides spp. and opens the way for new researches.