Detalhes bibliográficos
| Ano de defesa: |
2015 |
| Autor(a) principal: |
Oliveira, Stela Ramirez de
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| Orientador(a): |
Cunha, Luiz Carlos da
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| Banca de defesa: |
Cunha, Luiz Carlos da,
Oliveira, Flávia Neri Meira de,
Salazar, Vania Cristina Rodríguez,
Carvalho, Adryano Augustto Valladão de,
Santos, Mônica de Oliveira |
| Tipo de documento: |
Tese
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| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Goiás
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| Programa de Pós-Graduação: |
Programa de Pós-graduação em Ciências da Saúde (FM)
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| Departamento: |
Faculdade de Medicina - FM (RG)
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| País: |
Brasil
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| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://repositorio.bc.ufg.br/tede/handle/tede/4767
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Resumo: |
The objective of this study is to evaluate the inhibitory effects of derivatives of medicinal plants on CYP450 activity in rat liver microsomes by HPLC-PDA (high-performance liquid chromatography - photodiode array detection). Cerrado’s four plants were chosen for the inhibition test, because of the wide popular and traditional use: Stryphnodendron adstringens Mart (barbatimão), Copaifera langsdorffii Desf (copaíba), Lafoensia pacari A.St.-Hil. (pacari) and Pterodon emarginatus Vogel (sucupira), in addition to ten herbal medicines highly commercialized in Brazilian drugstores. To evaluate the inhibition of CYP3A, CYP2D6, CYP2C9, CYP1A2 four analytical methods were developed and validated by HPLC-PDA to quantify the substrates and metabolites. Afterward dried extracts of stem bark of Stryphnodendron adstringens and Lafoensia pacari, Copaifera langsdorffii oleoresin, Pterodon emarginatus essential oil and herbal medicines were incubated for one hour with rats liver microsomes to evaluate CYP3A inhibition (2.23 mg protein / mL liver microsome). All samples were dissolved in DMSO 5% and evaluated in three concentrations (high, medium, low). The analytical methods for quantification of biomarkers was appropriate after the validation for being linear, it did not show residual effect, it showed post-processing stability, and matrix effect within the ranges specified in the brazilian regulations. The method used to prepare the microsomal incubation and the method were suitable to assess the in vitro inhibition of CYP 3A. After analyzing the dried extracts and oil and oleoresin, was observed that they did not inhibit CYP3A. The absence of inhibitory activity of the extracts in oil and oleoresin isoenzyme CYP3A, although in vitro, adds relevant information about possible interference from non-traditional use of these plants on the metabolism of a large amount of drugs biotransformed by it. Among the tested herbal medicines, which showed greater inhibition of CYP3A was Monaless (O. sativa fermented by M. purpureus), which in higher concentration inhibited 31.94%, indicating that this product may lead to a possible interference when co-administered with drugs metabolized by CYP3A and may lead to drug interactions and adverse reactions. |
