Estudo da atividade citotóxica, genotóxica e apoptótica de novos complexos de ouro (III) de fluorquinolonas frente às células B16-F10, K562 e A20

• Ano de defesa: 2012
• Autor(a) principal: NUNES, Paula Roberta
• Orientador(a): LACERDA, Elisângela de Paula Silveira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Mestrado em Ciências Farmacêuticas
• Departamento: Ciências da Saúde - Farmácia
• País: BR
• Palavras-chave em Português: Complexo de Ouro; Antitumoral; Apoptose; Citotoxidade
• Palavras-chave em Inglês: Complex of Gold; Apoptosis; Cytotoxicity
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::CANCEROLOGIA
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tde/2114

The complex of gold K[Au(CN2)], for example, was introduced at the beginning of the twentieth century for the treatment of tuberculosis and then replaced for the gold thiolates (I), which were used in the decade of 30 for the treatment of Rheumatoid Arthritis. The exploration of gold complexes for the use as an anti-cancer agent, in an attempt to find a drug less toxic that cisplatin. The current study aimed to evaluate potential cytotoxic and genotoxic, as well as the mechanism of cell death of three new gold-based compounds, which is bioactive substance of synthetic origin, using different cell lines in vitro. In the cytotoxicity assay the effects of the three gold complexes were evaluated in four tumor cell lines (B16-F10, K562 and A20) and in two normal lines (L-929, MRC-5) through MTT test, the compounds at different concentrations (0.2, 2, 20, 50, 100, 200 μM) per 48 hours of treatment. The study was conducted to test electrophoresis on agarose gel at concentrations corresponding to IC50 of the strains tested. Were also performed cell cycle analysis and comet test. In statistical analysis for comparison between treated groups and control them, was used Anova, in a single criterion and Dunnet's Post-test (GraphPad Prism 3.02). The results obtained with MTT assay the tested strains MRC-5, L-929, B-16-F10, A20, K562, for the Spar Au compound was (104, 65.1, 45, 48.3, 61 2, respectively) for the Au Levo compound was (52.8, 181, 28.8, 48.9, 50, respectively) and the Au Nor compound was (65.1, 79.2, 26 6, 24.9, 55, respectively). After the screening of compounds, more specific tests were performed only with some strains that showed more promising results. For K562 cells and B16-F10 treated with Au Levo and Au Spar, respectively, observed that the test showed no degradation of DNA by apoptosis in the tests performed with 48 hours of treatment with the compounds. The line B16-F10 treated with the compound Au Spar did not significantly alter cell cycle kinetics and no reported significant damage to DNA after 48 hours of treatment. More specific tests have to be made in order to deepen the mechanism of cell death, since the tests were not enough to demonstrate how the compound is acting.