Detalhes bibliográficos
| Ano de defesa: |
2015 |
| Autor(a) principal: |
Nascimento, Jacqueline Barbosa
 |
| Orientador(a): |
Barrigossi, José Alexandre Freitas
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| Banca de defesa: |
Barrigossi, José Alexandre Freitas,
Fernandes, Paulo Marçal,
Albernaz, Karina Cordeiro,
Borga, Tereza Cristina de Oliveira,
Hirose, Edson |
| Tipo de documento: |
Tese
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| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Goiás
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| Programa de Pós-Graduação: |
Programa de Pós-graduação em Agronomia (EAEA)
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| Departamento: |
Escola de Agronomia e Engenharia de Alimentos - EAEA (RG)
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| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://repositorio.bc.ufg.br/tede/handle/tede/4586
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Resumo: |
The change in composition of the host-plants in the agricultural landscape can generate enough divergent natural selection to allow ecological adaptation that leads to specialization and subsequently to speciation in species of insect. The objective of this study was to determine the diversity of genetic and population structures of Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae), which was collected in two hostplants using microsatellite markers. Samples of larvae of this stem borer were collected on rice and sugarcane in different fields during the harvest of 2012/2013 in the states of Tocantins, Goiás, Mato Grosso, Mato Grosso do Sul, and Rio Grande do Sul. A total of 127 alleles were found distributed among the eight loci in seventeen populations, with a total of 426 individuals. The loci were highly polymorphic, with average of 15,87 alleles. The expected heterozygosity (HE) and observed (HO) were 0,579 and 0,350, respectively. A lower total number of alleles (35) was found in population of Morrinhos, as opposed to Formoso do Araguaia which has the largest (62). The same way, the smallest and the largest average number of alleles per locus (Am) were observed in these populations (4,38 and 7,75). The population from the Formoso do Araguaia showed the highest value for the allelic richness (6,82). In all populations, the observed heterozygosity (HO) was lower than expected (HE). The average polymorphic information content (PIC) in populations of D. saccharalis was 0,530. The intrapopulational fixation indexes (f) estimated for each population showed positive values and significantly different from zero with a average value of 0,403. The coefficient of inbreeding species was 0,399. The estimates FIT and FST (θ) values were 0,437 and 0,062 respectively. These global estimates indicated that there is genetic structure among these populations. Despite global estimates had indicated genetic structure, the structuring (θ) among of populations of the sugarcane was not significant; in contrast with the populations of the rice which showed θ values significant. There was a significant correlation between genetic and geographic distances estimated using the Mantel’s test (r=0,76; p= 0,0033), in which there is an increase in pattern of genetic distance with the increase of geographic distance in cluster analysis and population structures observed with the formation of the two groups of populations. The first was formed by populations from the state of Goiás and the second was composed of populations of Mato Grosso, Mato Grosso do Sul and Rio Grande do Sul. The population of Formoso do Araguaia sampled in rice showed in an intermediate position to the two groups formed. These results indicate that the genetic diversity of D. saccharalis is structured in space and the plant-host, in this study, did not have a key role in the process of genetic divergence populations. |
