Detalhes bibliográficos
| Ano de defesa: |
2014 |
| Autor(a) principal: |
Silva, Ágabo Macêdo da Costa e
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| Orientador(a): |
Martins, Regina Maria Bringel
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| Banca de defesa: |
Martins, Regina Maria Bringel,
Lopes, Carmen Luci Rodrigues,
Aires, Rodrigo Sebba |
| Tipo de documento: |
Dissertação
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| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Goiás
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| Programa de Pós-Graduação: |
Programa de Pós-graduação em Medicina Tropical e Saúde Pública (IPTSP)
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| Departamento: |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
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| País: |
Brasil
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| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://repositorio.bc.ufg.br/tede/handle/tede/3710
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Resumo: |
Hepatitis C represents a public health problem worldwide. Hepatitis C virus (HCV) is classified into seven genotypes and many subtypes. Besides their epidemiological importance, these genotypes have influence on the response to hepatitis C treatment, as well as other factors related to the virus and its host, such as polymorphisms upstream of interleukin (IL) 28B locus. Despite the importance of these factors in the response to treatment of hepatitis C, there are no data regarding the subject in the Midwest Region of Brazil. The present study aimed to identify the genotypes of HCV among patients attended at a reference laboratory in Goiânia-GO, and also to assess response to treatment of patients infected with genotype 1 with pegylated interferon (PEG-IFN) and ribavirin (RBV), with emphasis on the polymorphism upstream of IL28B gene (rs 12979860). For HCV genotyping, a cross-sectional study was conduct in an anti-HCV positive patients in a reference laboratory in Goiânia-GO, during in a period of 10 years (2003 to 2012) (n = 1300). From January/2012 to December/2013 (n = 101), a cohort was conduct among patients infected with HCV genotype 1 treated with PEG-IFN and RBV in order to evaluate treatment response. Patients were interviewed and blood samples collected for detection of viral RNA by polymerase chain reaction (PCR) with primers complementary to the region 5’ noncoding (NC) of HCV. All HCV RNA positive samples were genotyped by line probe assay – LiPA, and the samples typed as 1a/1b, 2 and 4 were sequenced in the NS5B region of the viral genome. The analysis of single nucleotide polymorphism (SNP) rs12979860 C/T was performed by restriction fragment length polymorphism (RFLP). Of the 1300 samples tested for HCV RNA, 894 were positive. Among these, genotypes 1 (76.3%), 2 (1.9%), 3 (21.6%) and 4 (0.2%) were found. These data show the predominance of genotype 1, followed by 3 and 2, and support the results of other studies in Goiânia. In addition, genotype 4 was found in the studied population. Regarding to SNP rs12979860 C/T, the TC genotype was the most common (57.4%), followed by CC (23.8%) and TT (18.8%). The rate of sustained virologic response (SVR) was 28.7%, being higher in individuals with the CC genotype (54.2%) when compared to those with CT and TT genotypes (22.4% and 15.8%, respectively). These results indicate that analysis of SNP rs12979860 is an important predictor of SVR following PEG-IFN and RBV treatment in patients infected with HCV genotype 1 in our region. |
