Detecção de Cryptosporidium spp. em amostras fecais de gatos (Catus felis domesticus) de Goiânia, Goiás

• Ano de defesa: 2017
• Autor(a) principal: Barrios, Leda Margarita Castaño
• Orientador(a): Jayme, Valéria de Sá
• Banca de defesa: Jayme, Valéria de Sá, Meireles, Marcelo Vasconcelos, Andrade, Maria Auxiliadora
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
• Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
• País: Brasil
• Palavras-chave em Português: C. felis; Criptosporidiose; Felinos; Nested PCR
• Palavras-chave em Inglês: C. feline; Nested PCR; Criptosporidiose; Feline
• Área do conhecimento CNPq: MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS)
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/7093

The purpose of this study was to detect the presence of Cryptosporidium spp. by the nested PCR in 95 samples of cat feces collected in four localities in Goiânia, Goiás, Brazil. In positive samples, we determined: (i) the frequency of Cryptosporidium spp., (ii) the species present, through BLAST sequences analysis, and (iii) risk factors (age, sex, and lifestyle of cats) using logistic regression and odds ratio (OR). We identified 17.9% (17/95) of samples were positive for Cryptosporidium spp. infection and, of these, 8,4% (8/95) were from males and 9,5% (9/95) from females. Among total samples, 7.4% (7/95) were from free-living animals and 10,5% (10/95) from non-free; 13,7% (13/95) were under six months of age and 4,2% (4/95) over six months of age. Regarding their origins, 7,4% (7/95) were from NGOs, 6,3% (6/95) from the University Veterinary Hospital, and 4,2 (4/95) from the premises of “Campus Samambaia”. Was detected circulating of C. felis 88,2 % (15/17), C. muris 5,9% (1/17) and Cryptosporidium sp. 5,9% (1/17) in the sampled animals. The only risk factor was age, for cats younger than six months (χ2 = 4,3, p= 0.04, OR = 3,4). This study demonstrated the existence of positive animals for Cryptosporidium spp. At least two species of Cryptosporidium circulate among the cats studied, affecting mainly cats under the age of six months.