Detalhes bibliográficos
| Ano de defesa: |
2019 |
| Autor(a) principal: |
Vasconcelos, Mariana Oliveira
 |
| Orientador(a): |
Lima, Eliana Martins
|
| Banca de defesa: |
Lima, Eliana Martins,
Arantes, Diego Antônio Costa,
Rocha, Thiago Lopes |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Goiás
|
| Programa de Pós-Graduação: |
Programa de Pós-graduação em Ciências Farmacêuticas (FF)
|
| Departamento: |
Faculdade Farmácia - FF (RG)
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://repositorio.bc.ufg.br/tede/handle/tede/9713
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Resumo: |
In this work the incorporation of polymeric nanoparticles containing lidocaine and chloramphenicol in biomembranes of chitosan, hyaluronic acid and glycerol was studied for the application of regenerative processes. The technique called guided tissue regeneration is used in the treatment of periodontal diseases. Two nanoparticle dispersions were prepared by the nanoprecipitation method, containing the drugs lidocaine and chloramphenicol, and characterized by the mean diameter, polydispersity index, encapsulation efficiency and in vitro release profile. Eight biomembranes of different compositions were divided into a group A (200 mg chitosan) and group B (70 mg chitosan), obtained by the casting method and characterized by visual aspect, thickness, mechanical properties, degradation assay, morphology, hydration profile, antimicrobial evaluation and cellular cytotoxicity. The nanoparticles of lidocaine and chloramphenicol nanoparticles had an average size of 200 nm with PdI of 0.2 and encapsulation efficiency of 77% and 82% for lidocaine and chloramphenicol, respectively. Drug releases occurred in 48 hours for nanoparticles containing lidocaine and 120 hours for nanoparticles containing chloramphenicol. Biomembranes showed significant differences in thickness. The tensile test showed that although the membranes do not present significant differences in the deformation and low resistance, they have enough mechanical resistance to act as a physical barrier. In the degradation assay the biomembranes M1 (group A) and M7 (group B) had the largest remaining mass after 28 days of testing with a 3 hour in vitro release of the nanoparticles by means of the biomembranes. The evaluation of antibacterial activity demonstrated that all bacteria were sensitive to formulations containing the antibiotic chloramphenicol and cytotoxicity tests indicated that biomembranes are safe for dental use. The results obtained showed that biomembranes incorporated with polymeric nanoparticles containing lidocaine and chloramphenicol are promising to be used as a physical barrier in the regenerative periodontal treatments, combining anesthesia, antimicrobial activity and tissue regeneration promoter. |
